16-101
Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate
clone 4G10®, Upstate®, from mouse
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
4G10®, monoclonal
Reattività contro le specie
vertebrates
Produttore/marchio commerciale
Upstate®
tecniche
immunoprecipitation (IP): suitable
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... PID1(55022)
Descrizione generale
Anti-Phosphotyrosine antibody (05-321), cross-linked to protein A agarose by dimethylpimelimidate
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Specificità
Recognizes tyrosine-phosphorylated proteins from all species.
Immunogeno
Phosphotyramine-KLH.
Applicazioni
Anti-Phosphotyrosine Antibody, clone 4G10, agarose conjugate is an antibody against Phosphotyrosine for use in IP.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
This product is useful for immunoprecipitation and immunoaffinity chromatography methods.
Suitable for immunoprecipitation and affinity purification.
Suitable for immunoprecipitation and affinity purification.
Caratteristiche e vantaggi
Format: Gel Immobilized
Qualità
Evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.
Immunoprecipitation Analysis:
10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
Immunoprecipitation Analysis:
10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
Descrizione del bersaglio
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Stato fisico
1 mg of Anti-Phosphotyrosine monoclonal antibody covalently coupled to 1 mL of protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.05% Kathon as a preservative, for a total volume of 2 mL. Liquid suspension.
Protein A purified
Stoccaggio e stabilità
Stable for 1 year at 2-8ºC from date of receipt.
Risultati analitici
Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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