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Supelco

Purospher® STAR RP-18 endcapped (3 µm) HPLC Columns

L × I.D. 100 mm x 2.1 mm Hibar® HR UHPLC Column

Sinonimo/i:

Purospher® STAR RP-18 endcapped (3µm) Hibar® HR 100-2.1

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About This Item

Codice UNSPSC:
41115709
NACRES:
SB.52

product name

Purospher® STAR RP-18 endcapped (3 µm), L × I.D. 100 mm x 2.1 mm Hibar® HR UHPLC column

Materiali

stainless steel column

Livello qualitativo

agenzia

suitable for USP L1

Nome Commerciale

Purospher®

Caratteristiche

endcapped

Confezionamento

1 ea of

Grado di funzionalizzazione

17% Carbon loading

Parametri

1000 bar pressure
65 °C max. temp.

tecniche

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

Lungh. × diam. int. colonna

10 cm × 2.1 mm

Area superficiale

330 m2/g

Matrice

fully porous particle

Gruppo funzionale matrice

C18 bonding phase

Dimensione particelle

3 μm

Dimensione pori

120 Å pore size

operating pH range

1.5-10.5

Tecnica di separazione

reversed phase
supercritical fluid (SFC)

Temperatura di conservazione

no temp limit

Descrizione generale

Purospher® STAR RP-18 endcapped, 3 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Purospher® STAR RP-18 endcapped, 3 µm Hibar® HR 100-2.1 is a ready to use UHPLC column suitable for connection to any UHPLC system providing a pressure stability of 1000 bar.The column dimension of 100 mm length x 2.1 mm I.D. is the optimal choice for fast and high resolution UHPLC separation of complex samples allowing significant solvent savings and improved sensitivity. A 2.1 mm I.D. column enables a more sustainable separations in comparison to 4, 4.6 or 3 mm I.D. column due to the use of significant less solvent for the chromatographic separation.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Applicazioni

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Caratteristiche e vantaggi

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Risultati analitici

Pressure: ≤ 120 bar
Theoretical Plates (N/m) (1,2-Benzanthracene): ≥ 130000
Symmetry (1,2-Benzanthracene): 0.8 - 1.4
Capacity factor (1,2-Benzanthracene): 7.0 - 10.0

Note legali

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articoli

Xanthine is a purine base found in most human body tissues and fluids as well as in other organisms. Methylated xanthines (methylxanthines), which include caffeine, paraxanthine, theobromine, and theophylline, commonly used for their effects as mild stiµlants and as bronchodilators, notably in the treatment of asthma symptoms. This application shows the efficient separation of several common xanthines and may be applied their analysis in any number of desired matrices.

Protocolli

broad spectrum insecticide, egradation product, phenylpyrazole pesticide, World Health Organization (WHO), HPLC, UV, testing of Fipronil and Fipronil sulfone in Food

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