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Key Documents

53829-U

Supelco

Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Sinonimo/i:

Colonna per HPLC Core-shell (SPP) Fused Core C18

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About This Item

Codice UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Colonna per HPLC Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

Materiali

stainless steel column

agenzia

suitable for USP L1

Nome Commerciale

Ascentis®

Caratteristiche

endcapped

Produttore/marchio commerciale

Ascentis®

Confezionamento

1 ea of

Parametri

60 °C temp. range
600 bar max. pressure (9000 psi)

tecniche

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

Lungh. × D.I.

15 cm × 4.6 mm

Area superficiale

135 m2/g

Impurezze

<5 ppm metals

Matrice

Fused-Core particle platform
superficially porous particle

Gruppo funzionale matrice

C18 (octadecyl) phase

Dimensione particelle

2.7 μm

Dimensione pori

90 Å

pH di lavoro

2-9

applicazioni

food and beverages

Tecnica di separazione

reversed phase

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Descrizione generale

Le colonne per HPLC Ascentis Express, grazie alla tecnologia delle particelle Fused-Core®, offrono l′alta velocità e l′elevata efficienza delle particelle di dimensioni inferiori a 2 μm, mantenendo ridotte nel contempo le contropressioni. La combinazione di alta efficienza e bassa contropressione è un grande vantaggio per tutti coloro che utilizzano la UPLC® (o altri sistemi a pressione ultra-alta) e l′HPLC convenzionale.
Per ulteriori informazioni su questa nuova tecnologia, si rimanda alla pagina Ascentis Express

Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

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Silvia Jakabová et al.
Journal of chromatography. A, 1232, 295-301 (2012-03-07)
Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a
Judy Stone
Methods in molecular biology (Clifton, N.J.), 1378, 301-320 (2015-11-26)
Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 %
Babu Rao Chandu et al.
SpringerPlus, 2(1), 194-194 (2013-06-07)
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human
Petra Šilarová et al.
Food chemistry, 237, 471-480 (2017-08-03)
The degradation of catechins and other phenolics in green tea infusions were monitored using fast HPLC/MS separation. The final separation was performed within 2.5min using Ascentis Express C18 column (50mm×2.1mm i.d.) packed with 2μm porous shell particles. Degradation was studied
Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within

Articoli

Compendial methods from the USP (United States Pharmacopeia) are widely used in pharmaceutical drug product and raw materials testing. However, not all methods in the USP use modern technologies. In chromatographic methods, it is not uncommon that older brands of columns are specified. Therefore, the USP methods are under continuous revision to improve existing procedures or to allow the user to obtain better results.

Apixaban is sold under the tradename Eliquis and at present no pharmacopeial monographs are available for Axpixaban using either an assay and/or organic impurity profiling method. A newly developed and validated HPLC-UV method using a column with C18 solid core particles is demonstrated.

Ginsenosides Separation in Ginseng. The HPLC method was first optimized using a ginsenoside standard mixture, and was then applied to a sample of American Ginseng root.

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

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Protocolli

This paper illustrates the setting up of a Lopinavir assay following European pharmacopoeia guidelines (10.2), and using a shorter column packed with smaller particles. The described assay saves valuable time and improves separation efficiency, which typically translates to better method performance.

This method is particularly useful in research into the role of individual bile acids as signaling molecules; suitable for clinical laboratories to investigate potential mechanisms linked to gut hormone profiles and glycemic control.

HPLC Analysis of Benzene and Deuterated Benzene on Ascentis® Express C18

Separation of Retinyl acetate, analytical standard; δ-Tocopherol, analytical standard; Ergocalciferol (D2), analytical standard; Cholecalciferol (D3), analytical standard; (±)-α-Tocopherol, analytical standard; Vitamin E acetate, analytical standard; Phylloquinone (K1), analytical standard; Retinol palmitate, analytical standard.

Chromatograms

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