14-484-M

PKCα Protein, active, 10 µg

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

• Sinonimo/i: PRKCA, Protein kinase C alpha type
• Codice UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Livello qualitativo: 100
• Ricombinante: expressed in baculovirus infected Sf21 cells
• Nome Commerciale: Upstate^®
• Attività specifica: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• Durata: 1 yr
• PM: M_w 78 kDa
• Purificato mediante: (Ni²⁺/NTA-agarose)
• Reattività contro le specie: human
• Produttore/marchio commerciale: Upstate^®
• tecniche: activity assay: suitable (kinase)
• Numero d’accesso: (FUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. ); NM_002737.2
• N° accesso NCBI: NM_002737.2
• N° accesso UniProt: P17252
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... PRKCA(5578)

Descrizione

Descrizione generale

Research area: Apoptosis

Protein Target: PKCα

Target Sub-Family: AGC

Recombinant, full-length human PKCα, containing N-terminal His6 tag

Applicazioni

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

Azioni biochim/fisiol

Protein kinase C-α (PKCα), an isoform of protein kinase C, exhibits an anti-apoptotic and pro-proliferative function. It is commonly upregulated in tumor cells, and its depletion has been associated with apoptosis induction. The anti-apoptotic effects of PKCα are mediated through mechanisms such as phosphorylation of the anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) and activation of the pro-survival protein kinase AKT. The inhibition of PKCα leads to the activation of a PKCδ-dependent apoptotic pathway in salivary epithelial cells. PKCα regulates cell cycle progression in glioma cells by increasing the expression of p21 cyclin-dependent kinase inhibitor 1 (p21 (Waf1/Cip1)), a protein involved in cell cycle regulation.

Confezionamento

Also available in 10 μg size and in bulk

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-484M when ordering the 250 µg size.

Qualità

routinely evaluated by phosphorylation of Histone H1.

Descrizione del bersaglio

PKCα

Stato fisico

Ni²⁺/NTA-agarose

Stoccaggio e stabilità

1 year at -70°C

Altre note

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H317
• Consigli di prudenza: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classi di pericolo: Skin Sens. 1
• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°C): Not applicable