Passa al contenuto
Merck
Tutte le immagini(1)

Documenti fondamentali

1.01597

Sigma-Aldrich

AFB-Fluor phenol-free

Staining kit for the examination of acid-fast bacteria with fluorescence microscopy (Auramin-Rhodamine staining)

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
41116130
NACRES:
NA.74

Livello qualitativo

IVD

for in vitro diagnostic use

tecniche

microbe id | staining: suitable

applicazioni

clinical testing
diagnostic assay manufacturing
hematology
histology

Metodo di rivelazione

fluorometric (microscopy)

Temperatura di conservazione

15-25°C

Descrizione generale

The decisive feature of the AFB-Fluor phenol-free for detection of aldehyde and mucosubstances, is the presence of fluorescence dyes in the staining solution. This enables the swift and secure identification of the acid-fast bacteria under the fluorescence microscope. As it has become an established procedure alongside Ziehl-Neelson staining (AFB-Color modified), we provide three ready-to-use solutions in our AFB-fluor, phenol-free staining kit. Of these, the first solution contains the two fluorescent dyes, Rhodamine B – staining red - and Auramine O – staining yellowish-green – which can be used depending on the filter combination of the microscope. Here, the auramine-rhodamine staining solution is prepared without phenol and the efficient penetration of the dyes into the target structures is achieved through a phenol substitute that is more user- and eco-friendly than phenol. Further, the kit supplies a decolorization solution and a potassium permanganate solution that turns the background uniformly black, suppressing troublesome intrinsic fluorescence of the specimen. If required, double staining with AFB-Color modified (Product number 1.00497) (ZN) is possible. The AFB-fluor, phenol-free-kit, modified is designed to be used for smears, thougt it can also be used for paraffin sections.
The kit is sufficient for up to 60-65 preparations and is an IVD / CE registered product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Risultati analitici

Suitability for microscopy: passes test
Mycobacteria: red-orange/yellow-green
Background: dark

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Aquatic Chronic 2 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.