07-595

Anti-acetyl-Histone H4 (Lys12) Antibody

serum, Upstate^®

• Sinonimo/i: H4K12Ac, Histone H4 (acetyl K12)
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: serum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: Saccharomyces cerevisiae, human
• Produttore/marchio commerciale: Upstate^®
• tecniche: ChIP: suitable (ChIP-seq); dot blot: suitable; western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NM_175054
• N° accesso UniProt: P62805; Q6DRA9
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: acetylation (Lys12)
• Informazioni sul gene: human ... HIST2H4B(554313)

Descrizione

Descrizione generale

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specificità

Broad species cross-reactivity expected

Recognizes Histone H4 acetylated on lysine 12.

Immunogeno

peptide containing the sequence (LG_AcKGG) corresponding to lysine 12 acetylation of yeast Histone H4.

Applicazioni

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-acetyl-Histone H4 (Lys12) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, DB, WB, ChIP-seq to detect acetyl-Histone H4 (Lys12) also known as H4K12Ac, Histone H4 (acetyl K12).

Qualità

routinely evaluated by immunoblot on acid extracted proteins from HeLa cells

Descrizione del bersaglio

10 kDa

Stato fisico

Depleted rabbit antiserum in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Immunodepleted Serum

Stoccaggio e stabilità

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Risultati analitici

Control
Acid extracted proteins from HeLa cells treated with sodium butyrate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1