07-571-I

Anti-nNOS/NOS I Antibody

0.5 mg/mL, from rabbit

• Sinonimo/i: Nitric oxide synthase, brain, bNOS, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, N-NOS, nNOS, Peptidyl-cysteine S-nitrosylase NOS1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: mouse, rat
• Concentrazione: 0.5 mg/mL
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• N° accesso NCBI: NP_434686
• N° accesso UniProt: P29476
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Nos1(18125) ; rat ... Nos1(24598)

Descrizione

Descrizione generale

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. nNOS/NOS I, also known as NC-NOS, or NOS type I, or Neuronal NOS, or Peptidyl-cysteine S-nitrosylase NOS1 and bNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NOS I has been suggested to have nitrosylase activity and to mediate cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

Immunogeno

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to the near N-terminus of rat nNOS/NOS I.

Applicazioni

Anti-nNOS/NOS I Antibody is an antibody against nNOS/NOS I for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected nNOS/NOS I in rat cerebellum and cerebral cortex tissues, and mouse hippocampus and thalamus tissues.

Research Category
Apoptosis & Cancer

Research Sub Category
Neurodegenerative Diseases

Qualità

Evaluated by Western Blotting in rat brain microsomal preparation.

Western Blotting Analysis: A 1:500 dilution of this antibody detected nNOS/NOS I in 10 µg of rat brain microsomal preparation.

Descrizione del bersaglio

~165 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Linkage

Replaces: 07-571

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable