07-329-AF488

Anti-acetyl-Histone H4 (Lys16) Antibody, Alexa Fluor^™ 488

from rabbit, ALEXA FLUOR^™ 488

• Sinonimo/i: Histone H4, acetyl-Histone H4 (Lys16), AlexaFluor^™ 488
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Coniugato: ALEXA FLUOR^™ 488
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: mouse, human
• Reattività contro le specie (prevista in base all’omologia): rat (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable
• N° accesso NCBI: NP_009563.1
• N° accesso UniProt: P02309
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: acetylation (Lys16)
• Informazioni sul gene: human ... H4C1(8359) ; mouse ... H4C1(326619)

Descrizione

Descrizione generale

Histone H4 (UniProt P02309) is encoded by the HHF1 (ORF YBR0122; Gene ID 852294) and HHF2 (ORF N2752; Gene ID 855701) genes in yeast species. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specificità

Broad species cross-reactivity is expected.

H4K16ac specificity of the unconjugated antibody (Cat. No. 07-329) has been confirmed using the Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and the Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Immunogeno

Epitope: H4K16ac

Ovalbumin-conjugated, synthetic peptide (…GAAcKRH …) in which AcK corresponds to residue 16 of yeast histone H4.

Applicazioni

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected acetyl-Histone H4 (Lys16) in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-329) is shown to be suitable also for ChIP, ChIP-seq, Western blotting, multiplex assay, dot blot applications.

This Anti-acetyl-Histone H4 (Lys16) Antibody, AlexaFluor 488 is validated for use in Immunocytochemistry for the detection of acetyl-Histone H4 (Lys16).

Qualità

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected acetyl-Histone H4 (Lys16) in HeLa cells.

Descrizione del bersaglio

~10 kDa observed

Altre note

Concentration: Please refer to lot specific datasheet.

Note legali

ALEXA FLUOR is a trademark of Life Technologies

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable