06-913-I

Anti-Sin3A Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Paired amphipathic helix protein Sin3a, Histone deacetylase complex subunit Sin3a, Transcriptional corepressor Sin3a, Sin3A
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• application: ICC; WB
• Reattività contro le specie: human, mouse
• tecniche: immunocytochemistry: suitable; western blot: suitable

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human, mouse
• Reattività contro le specie (prevista in base all’omologia): rat (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_035508
• N° accesso UniProt: Q60520
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SAP30(8819) ; mouse ... Sin3A(20466)

Descrizione

Descrizione generale

Paired amphipathic helix protein Sin3a (UniProt Q60520; also known as Histone deacetylase complex subunit Sin3a, mSin3a, Transcriptional co-repressor Sin3a) is encoded by the Sin3A (also known as mSin3A, AW553200, Kiaa4126, mKIAA4126) gene (Gene ID 8819) in murine species. The transcription regulator Sin3a is a scaffold protein that interacts with various proteins to form specialized complexes that mediate different biological processes, including chromatin modification, embryonic development, DNA repair, mitochondrial metabolism, and gene transcription. SIN3a harbors four paired α-helices (PAH), a histone interaction domain, and a highly conserved region. The histone interaction domain mediates HDACs binding, whereas the highly conserved region interacts with proteins necessary for repression, such as the nuclear receptor hormone co-repressor Alien. The four PAHs represent the main interacting domains of SIN3a and mediate the interactions with a wide variety of DNA-binding transcription factors. Members of the Mad family of repressor proteins, for example, specifically interact with the PAH2 through their Sin3 interacting domain (SID). Although widely studied for its involvement in transcriptional repression, Sin3a is shown to be essential for aryl hydrocarbon receptor-mediated induction of gene transcription.

Immunogeno

Epitope: N-terminus

KLH-conjugated peptide (KRRLDDQESPVYAAQQRR-C) corresponding to amino acids 2-19 of mouse Sin3A.

Applicazioni

Anti-Sin3A Antibody is an antibody against Sin3A Antibody for use in Western Blotting and Immunocytochemistry.

Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected Sin3A in HeLa, A431, HUVEC, and NIH/3T3 cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Qualità

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.05 µg/mL of this antibody detected Sin3A in 10 µg of HeLa nuclear extract.

Descrizione del bersaglio

~160 kDa observed

Linkage

Replaces: 06-913

Stato fisico

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable