06-720

Anti-HDAC1 Antibody

Upstate^®, from rabbit

• Sinonimo/i: histone deacetylase 1, reduced potassium dependency, yeast homolog-like 1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: rat, human, mouse
• Confezionamento: antibody small pack of 25 μg
• Produttore/marchio commerciale: Upstate^®
• tecniche: activity assay: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_004955
• N° accesso UniProt: Q13547
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... HDAC1(3065) ; mouse ... Hdac1(433759) ; rat ... Hdac1(297893)

Descrizione

Descrizione generale

Mammalian HADC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all are related to the yeast transcriptional regulator Rpd3p, are identified as histone deacetylases. The deacetylation of histones is associated with transcriptional silencing.

Specificità

Oher species cross-reactivity not tested.

Recognizes and is specific for HDAC1, Mr 65 kDa. May detects a non-specific protein at ~35 kDa.

Immunogeno

6X-His-tagged fragment representing amino acids 53-482 of mouse histone deacetylase 1 (HDAC1).

Applicazioni

Immunoprecipitation:
5 μg of a previous lot immunoprecipitated HDAC1 from 500 μg of 3T3/A31 RIPA lysate.

Immunocytochemistry:
This antibody has been reported to immunostain HDAC1 in 1% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 3T3 cells.

HDAC Activity:
This antibody has been reported to immunoprecipitate active HDAC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-HDAC1 Antibody (Rabbit Polyclonal Antibody) validated in EA, ICC, IP, WB to detect HDAC1 also known as histone deacetylase 1.

Qualità

Routinely evaluated by western blot on RIPA lysates from 3T3/A31 cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected HDAC1 in RIPA lysates from 3T3/A31 cells.

Descrizione del bersaglio

65 kDa

Stato fisico

Format: Purified

Protein A chromatography

Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, containing 0.05% sodium azide. Frozen at -20ºC.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable