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Merck
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05-844

Sigma-Aldrich

Anti-PLK1 Antibody, clone 35-206

clone 35-206, Upstate®, from mouse

Sinonimo/i:

Polo-like kinase 1, Serine/threonine-protein kinase 13, cell cycle regulated protein kinase, polo (Drosophia)-like kinase, polo like kinase, polo like kinase, polo-like kinase (Drosophila), polo-like kinase 1 (Drosophila)

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

100
400

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

35-206, monoclonal

Reattività contro le specie

rat, mouse, human

Produttore/marchio commerciale

Upstate®

tecniche

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2b

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... PLK1(5347)
mouse ... Plk1(18817)

Descrizione generale

Polo-like Kinase 1 (PLK-1) is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Expressed PLK-1 protein accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.

Specificità

PLK1

Immunogeno

6His-tagged full length fusion protein corresponding to human polo-like kinase 1 (PLK1). Also called serine-threonine protein kinase PLK.

Applicazioni

Anti-PLK1 Antibody, clone 35-206 detects level of PLK1 & has been published & validated for use in WB, IP, IC, IH.
Immunoprecipitation: An independent laboratory has reported that a previous lot of this antibody can be used to immunoprecipitate PLK1.

Immunohisto/immunocytochemistry: An independent laboratory reported that 1 μg/mL of a previous lot of this antibody showed positive immunostaining for PLK1 in paraformaldehyde treated, Triton X-100 fixed cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualità

Evaluated by western blot on RIPA lysates from 3T3/A31 cells.

Western Blot Analysis: 0.5-2 μg/mL of this antibody detected PLK1 in RIPA lysates from 3T3/A31 cells.

Descrizione del bersaglio

64 kDa

Stato fisico

Format: Purified
Protein A purified
Protein A purified mouse IgG2b in buffer containing 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol. Store at -20°C.

Stoccaggio e stabilità

Stable for 1 year at from date of receipt.

Risultati analitici

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Matthew K Martz et al.
Molecular biology of the cell, 24(18), 2785-2794 (2013-07-26)
Proper completion of mitosis requires the concerted effort of multiple RhoGEFs. Here we show that leukemia-associated RhoGEF (LARG), a RhoA-specific RGS-RhoGEF, is required for abscission, the final stage of cytokinesis, in which the intercellular membrane is cleaved between daughter cells.
Christian D Fingas et al.
Hepatology (Baltimore, Md.), 58(4), 1362-1374 (2013-05-25)
Cholangiocarcinoma (CCA) cells paradoxically express the death ligand tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and thus rely on potent survival signals to circumvent cell death by TRAIL. Hedgehog (Hh) signaling is an important survival pathway in CCA. Herein, we further
BRCA1 downregulates the kinase activity of Polo-like kinase 1 in response to replication stress.
Zou, J; Rezvani, K; Wang, H; Lee, KS; Zhang, D
Cell Cycle null
Neuronal c-Abl activation leads to induction of cell cycle and interferon signaling pathways.
Schlatterer, SD; Suh, HS; Conejero-Goldberg, C; Chen, S; Acker, CM; Lee, SC; Davies, P
Journal of Neuroinflammation null
Sol-Bi Shin et al.
Therapeutic advances in medical oncology, 11, 1758835919846375-1758835919846375 (2019-06-04)
Despite the clinical success of taxanes, they still have limitations, such as chemoresistance. To overcome the limitations of paclitaxel, genetic alterations and targeting effects of altered genes were observed in paclitaxel-resistant cancer. Because paclitaxel-resistant cancer shows high levels of Plk1

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