05-629-IM

Anti-Histone H1o/H5 Antibody, clone 3H9

ascites fluid, clone 3H9, from mouse

• Sinonimo/i: Histone H5, Histone H1o
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: ascites fluid
• Tipo di anticorpo: primary antibodies
• Clone: 3H9, monoclonal
• Reattività contro le specie: human, chicken
• Confezionamento: antibody small pack of 25 μL
• tecniche: ChIP: suitable (ChIP-chip); flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_001038138
• N° accesso UniProt: P02259
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... H1-0(3005)

Descrizione

Descrizione generale

Histone H1/H5 (UniProt: P02259) are linker histones that are involved in the organization and maintenance of chromatin higher order structure and they play an active role in the control of DNA replication and cell proliferation. Linker histones are essential for chromatin filament formation, and they play key roles in the regulation of gene expression. Histone H1o is a histone H5-like protein found in mammalian species. It is present in the linker region of chromatin and replaces H1 there. Up to one third of the H1 can be replaced in some tissues. Histone H5 performs the same function as H1, being necessary for the condensation of nucleosome chains into higher order structures, and replaces histone H1 in certain cells. Histone H5 is found in erythroid cells in avian species. Histone H5 consists of a central globular domain (GH5) that is essential for nucleosome binding and is flanked by basic N- and C-terminal tails. It is reported that binding of either histone H5 or GH5 to nucleosomes protects an additional 20 bp of linker DNA from micrococcal nuclease digestion. Globular domain of linker histone H5 (GH5) contains three distinct DNA-binding sites, through which GH5 contacts the DNA at the nucleosome dyad and the linker DNA strands entering and exiting the nucleosome. (Ref.: Segers, A et al (1991). J. Biol. Chem. 266(3); 1502-1508; Fan, L., and Roberts, VA (2006). Proc. Natl. Acad. Sci. USA. 113 (22); 8384 8389; Smith, BH and Jones, EW (1980). Nucleic Acids Res. 8(24); 6069-6079).

Specificità

Clone 3H9 detects Histone H1o/H5 in human and chicken species. it targets an epitope within 46 amino acids from the N-terminal region.

Immunogeno

Native histone H5 protein purified from chicken erythrocytes.

Applicazioni

Anti-Histone H1o/H5, clone 3H9 , Cat. No. 05-629-I, is a highly specific mouse monoclonal antibody that targets Histone H5 and has been tested in Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Western Blotting.

Western Blotting Analysis: A representative lot detected Histone H1o/H5 in Western Blotting applications (Torres, C.M., et. al. (2016). Science. 353(6307)).

Chromatin Immunoprecipitation Analysis: A representative lot detected Histone H1o/H5 in Chromatin Immunoprecipitation applications (Torres, C.M., et. al. (2016). Science. 353(6307)).

Flow Cytometry Analysis: A representative lot detected Histone H1o/H5 in Flow Cytometry applications (Torres, C.M., et. al. (2016). Science. 353(6307)).

Immunocytochemistry Analysis: A representative lot detected Histone H1o/H5 in Immunocytochemistry applications (Torres, C.M., et. al. (2016). Science. 353(6307)).

Immunofluorescence Analysis: A representative lot detected Histone H1o/H5 in Immunofluorescence applications (Torres, C.M., et. al. (2016). Science. 353(6307)).

Qualità

Evaluated by Western Blotting in HeLa cells acid extract.

Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Histone H1o in 10 µg of HeLa cells acid extract.

Descrizione del bersaglio

~30 kDa observed; 20.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Mouse monoclonal antibody in ascites without preservatives.

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable