05-443

Anti-VEGF Antibody, clone JH

clone JH, Upstate^®, from mouse

• Sinonimo/i: Vascular permeability factor, vascular endothelial growth factor, vascular endothelial growth factor A, vascular endothelial growth factor isoform VEGF165
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100; 400
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: JH, monoclonal
• Reattività contro le specie: rat, mouse, rabbit, human
• Produttore/marchio commerciale: Upstate^®
• tecniche: immunohistochemistry: suitable (paraffin)
• Isotipo: IgG1κ
• Compatibilità: not suitable for Western blot
• N° accesso NCBI: NM_003376
• N° accesso UniProt: P15692
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... VEGFA(7422)

Descrizione

Descrizione generale

The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.

Specificità

Other species not tested.

Recognizes 34-54 kDa isoforms of Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF).

Immunogeno

Recombinant human VEGF121. Clone JH121

Applicazioni

Immunohistochemistry: This lot of antibody detected VEGF in formalin fixed, paraffin embedded, human.tissues, using a 1:50 dilution incubated overnight at 4°C.

Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

This Anti-VEGF Antibody, clone JH is validated for use in IH(P) for the detection of VEGF.

Qualità

Biological Blockade: Incubation of HUVEC cells with 5 µg/mL of a previous lot and 5 ng/mL of VEGF (Catalog # 01-185) has shown growth inhibition of 20-70%.

Descrizione del bersaglio

34-54 kDa isoforms

Stato fisico

Format: Purified

Protein G Chromatography

Purified mouse monoclonal IgG1k in buffer containing 10 mM PBS, pH 7.4.

Stoccaggio e stabilità

Stable for 1 year at from date of receipt.

Risultati analitici

Control
Expressed in most tissue, including Pancreas, Kidney, Adenocarcinoma.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable