05-1923

Anti-phospho-Threonine Antibody, clone 20H6.1

clone 20H6.1, from mouse

• Sinonimo/i: Threonine
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Tipo di anticorpo: primary antibodies
• Clone: 20H6.1, monoclonal
• Reattività contro le specie: mouse, human
• Reattività contro le specie (prevista in base all’omologia): vertebrates (based on 100% sequence homology), all
• tecniche: immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG2bκ
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pThr)

Descrizione

Descrizione generale

Protein phosphorylation is normally associated with protein modification in signaling pathways and may be useful in pathophysiology and treatment of major neuropsychiatric disorders. Antibodies against phospho-threonine have been used to establish stability of the phosphorylated forms of proteins that may be involved in many different types of signaling. Phospho-threonine antibody is used to measure phosphorylation levels of PSII reaction center proteins D1, D3 and chlorophyll a binding protein CP43.

Immunogeno

KLH-conjugated linear peptide corresponding to Threonine.

Applicazioni

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Threonine from 100 µg of A431 cells stimulated with calyculin/okadaic acid.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Threonine in A431, NIH/3T3, and HeLa cells.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

This phospho-Threonine antibody is validated for use in WB & ICC for the detection of the phospho-Threonine protein.

Qualità

Evaluated by Western Blot in calyculin/okadaic acid treated and untreated A431 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Threonine on 10 µg of calyculin/okadaic acid treated and untreated A431 cell lysates.

Descrizione del bersaglio

Various observed

Stato fisico

Format: Purified

Protein G

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Calyculin/okadaic acid treated and untreated A431 cell lysates.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable