05-1469

Anti-NFĸB Antibody, p65, clone 1G10.2

clone 1G10.2, from mouse

• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 1G10.2, monoclonal
• Reattività contro le specie: bovine, human, mouse, rat
• tecniche: immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_068810.2
• N° accesso UniProt: P19838
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... RELA(5970)

Descrizione

Descrizione generale

NFĸB (Nuclear Factor kappa B) represents a group of transcription factors that are structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel. The five members in mammals includes Rel (cRel), RelA (p65), RelB, NFĸB1 (p50 and its precursor p105), and NFĸB2 (p52 and its precursor p100)1, 2. NFĸB/Rel proteins exist as homo- or heterodimer complexes.
In a majority of unstimulated cells, the NFĸB transcription factors are bound to the inhibitory IĸB proteins and retained in the cytoplasm in their inactive form. Upon stimulation by multiple inducers, IĸBα is rapidly phosphorylated and degraded. This results in the release of the NFĸB complex, most commonly the p105/p65 heterodimer. The p105 subunit is cleaved into its active p50 form. This cleavage exposes the NLS sequence on the p50 subunit. The p50/p65 heteroduplex then translocates to the nucleus where it activates gene transcription.

Specificità

Detects NFĸB p65.

Expected to cross-react with bovine based on complete sequence homology.

Other species have not been tested.

Immunogeno

Epitope: NLS

Synthetic peptide corresponding to the NLS region of human NFĸB p65.

Applicazioni

Anti-NFĸB Antibody, p65, clone 1G10.2 detects level of NFĸB & has been published & validated for use in WB, IF & IC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualità

Routinely evaluated by Western Blot.

Western Blot Analysis:
This lot detected NFB, p65 at 1:1,000 dilution in Jurkat cell lysate resolved via SDS-PAGE and transferred to PVDF.

Descrizione del bersaglio

65 kDa

Stato fisico

Format: Purified

Protein A Purified

Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4) with 150mM NaCl and 0.05% NaN3.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable