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Key Documents

05-1109

Sigma-Aldrich

Anti-Pro-Insulin C-Peptide Antibody, clone C-PEP-01

clone C-PEP-01, from mouse

Sinonimo/i:

CD220 antigen, insulin receptor

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

C-PEP-01, monoclonal

Reattività contro le specie

human

tecniche

immunohistochemistry: suitable (paraffin)

Isotipo

IgG1

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... INS(3630)

Descrizione generale

Pro-Insulin consists of the three parts: C-peptide and two long strands of amino acids (alpha and beta chains; later become linked together to form the Insulin molecule). From every molecule of Pro-Insulin, one molecule of Insulin plus one molecule of C-peptide are produced. C-peptide is released into the blood stream in equal amounts to Insulin.

Specificità

No cross-reactivity with Insulin or other peptide hormones or proteins was observed.
The antibody C-PEP-01 reacts specifically with C-peptide, a part of the Pro-insulin molecule.

Immunogeno

Synthetic C-peptide of human pro-insulin conjugated to bovine serum albumin.

Applicazioni

Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling
This Anti-Pro-Insulin C-Peptide Antibody, clone C-PEP-01 is validated for use in IH(P) for the detection of Pro-Insulin C-Peptide.

Qualità

Evaluated by Immunohistochemistry in normal human pancreas.
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected insulin in human normal pancreas tissue.

Descrizione del bersaglio

Approx. 12 kDa

Linkage

Replaces: CBL94

Stato fisico

Format: Purified
Mouse monoclonal IgG1 in phosphate buffered saline, pH7.4, and 15 mM sodium azide.
Sequential precipitation with caprylic acid and ammonium sulfate.

Stoccaggio e stabilità

Store at 2-8°C and use within 1 year from date of receipt.

Risultati analitici

Control
Human normal pancreas tissue

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Sapna Puri et al.
Nature communications, 9(1), 485-485 (2018-02-06)
Pancreatic β cells are highly specialized to regulate systemic glucose levels by secreting insulin. In adults, increase in β-cell mass is limited due to brakes on cell replication. In contrast, proliferation is robust in neonatal β cells that are functionally
Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells.
Yamada, Mitsutoshi, et al.
Nature (2014)
Haiqing Hua et al.
The Journal of clinical investigation, 123(7), 3146-3153 (2013-06-20)
Diabetes is a disorder characterized by loss of β cell mass and/or β cell function, leading to deficiency of insulin relative to metabolic need. To determine whether stem cell-derived β cells recapitulate molecular-physiological phenotypes of a diabetic subject, we generated
Bipasha Bose et al.
Methods in molecular biology (Clifton, N.J.), 1341, 257-284 (2015-03-19)
Since the advent of pluripotent stem cells, (embryonic and induced pluripotent stem cells), applications of such pluripotent stem cells are of prime importance. Indeed, scientists are involved in studying the basic biology of pluripotent stem cells, but equal impetus is

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