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Key Documents

803340

Sigma-Aldrich

Sulfo-SDA (Sulfo-NHS-Diazirine) (sulfosuccinimidyl 4,4′-azipentanoate)

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About This Item

Formula empirica (notazione di Hill):
C9H10N3Na7S
Peso molecolare:
353.19
Numero MDL:
Codice UNSPSC:
12352200
ID PubChem:
NACRES:
NA.22

Saggio

≥90%

Livello qualitativo

Forma fisica

powder

PM

327.25

Impiego in reazioni chimiche

reagent type: cross-linking reagent

Condizioni di stoccaggio

desiccated

Solubilità

water: soluble

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

Stringa SMILE

O=C(C(S(=O)([O-])=O)C1)N(OC(CCC2(N=N2)C)=O)C1=O.[Na+]

InChI

1S/C9H11N3O7S.Na/c1-9(10-11-9)3-2-7(14)19-12-6(13)4-5(8(12)15)20(16,17)18;/h5H,2-4H2,1H3,(H,16,17,18);/q;+1/p-1
KTYCFZFVXSHAGH-UHFFFAOYSA-M

Categorie correlate

Descrizione generale

Succinimidyl-diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions, and these SDA reagents extend the efficiency and range of interactions that can be explored by this approach.

Caratteristiche e vantaggi

  • Water soluble—solubility in aqueous solutions improved by a sulfonate group
  • Heterobifunctional—NHS ester group reacts with primary amines at pH 7 to 9 to form covalent amide bonds; diazirine (azipentanoate) group reacts efficiently with any amino acid side chain or peptide backbone upon activation with long-wave UV light (330-370 nm)
  • Controllable—two-step chemical crosslinking is activated using common laboratory UV lamps
  • Easy to use—these crosslinkers are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark
  • Better than aryl azides—the diazirine photoreactive group has better photostability in normal light than phenyl azide groups of traditional photoreactive crosslinkers, yet the diazirine group is more efficiently activated by long-wave UV light

Avvertenza

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Photoactivatable crosslinking sugars for capturing glycoprotein interactions.
Yoshihito Tanaka et al.
Journal of the American Chemical Society, 130(11), 3278-3279 (2008-02-26)
Monika Suchanek et al.
Nature methods, 2(4), 261-267 (2005-03-23)
Protein-protein interactions are the key to organizing cellular processes in space and time. The only direct way to identify such interactions in their cellular environment is by photo-cross-linking. Here we present a new strategy for photo-cross-linking proteins in living cells.

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