SAE0198

Calprotectin, human recombinant

Stabilized heterodimer of fusion tagged S100A9 and S100A8

• Synonyme(s) : MRP8/14 and S100A8/A9 complex
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 200
• Pureté: ≥90% (SDS-PAGE)
• Forme: lyophilized powder
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

This recombinant human calprotectin is a heterodimer composed of tagged versions of both S100A8 and S100A9, where a 6xHis-SUMO tag is added to the N-terminus of S100A9, and a 6xHIS tag is added to the N-terminus of S100A8. This tagged version was found to have enhanced stability as compared to untagged versions of this protein. Removal of the tags can be achieved using HRV-3C protease (SAE0045 or SAE0110).

Application

Fecal Calprotectin is often used as a biomarker to identify inflammation in gastrointestinal disease¹. This product is a recombinant version of the human protein found endogenously.

Actions biochimiques/physiologiques

Calprotectin is a calcium and zinc binding protein that belongs to the S100 family. It is a heterodimer protein composed of S100A8 and S100A9.¹ The protein is expressed in myeloid cells and in circulating monocytes and neutrophils, where it accounts for 60% of the cytosolic protein content. Calprotectin was found to play major roles in inflammation and the immune response by activation of NF-kB pathway via TLR4 and AGER,² and its antimicrobial activity possibly through chelating of Zn.³ High concentrations of calprotectin are found and serve as a biomarker in rheumatoid, cystic fibrosis, inflammatory bowel disease and in Crohn′s disease.⁴ A meta-analysis also identified calprotectin as a potential biomarker for COVID-19.⁵

Forme physique

The product is lyophilized from a sterile 0.2 µm filtered solution containing 12.5 mM Tris-Cl (pH 8.0), 150 mM NaCl, and 1 mM DTT.

Notes préparatoires

Reconstitute lyophilized vial in DMEM or in PBS to a concentration up to 500 µg/mL. Spin down to collect material. Reconstituted product should be stored in working aliquots at –20 °C. Do not store in a frost-free freezer. Avoid freeze-thaw cycles.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H319
• Conseils de prudence: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Classification des risques: Eye Irrit. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1