S8160
Superoxide Dismutase from bovine liver
lyophilized powder, ≥1500 units/mg protein
Synonyme(s) :
SOD, Superoxide: superoxide oxidoreductase
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About This Item
Forme
lyophilized powder
Activité spécifique
≥1500 units/mg protein
Poids mol.
32.5 kDa
Composition
Protein, ≥70% biuret
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
cow ... SOD1(281495)
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Description générale
Research area: Cell signalingSuperoxide Dismutase (SOD), a low molecular weight protein that is found in all aerobic cells of microorganisms, plants, and animals. It exists as three distinct families such as manganese SOD, copper–zinc SOD, and extracellular SOD.
Application
Superoxide dismutase from bovine liver has been used in a study to determine that hypercholesterolemia increases endothelial superoxide anion production. Superoxide dismutase from bovine liver has also been used in a study to investigate diazo coupling, subunit interactions, and electrophoretic variants of bovine erythrocyte superoxide dismutase. It has also been used as a component of the assay buffer in thequantification of reactive oxygen species (ROS) by O2k fluorometry.
Actions biochimiques/physiologiques
Superoxide Dismutase catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It plays a critical role in the defense of cells against the toxic effects of oxygen radicals. It competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.
Définition de l'unité
One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.
Forme physique
Lyophilized powder containing potassium phosphate buffer salts
Remarque sur l'analyse
For assay method, see McCord, J.M. and Fridovich, I., J. Biol. Chem., 244, 6049 (1969).
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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S D Yan et al.
The Journal of biological chemistry, 269(13), 9889-9897 (1994-04-01)
Attack by reactive oxygen intermediates, common to many kinds of cell/tissue injury, has been implicated in the development of diabetic and other vascular diseases. Such oxygen-free radicals can be generated by advanced glycation end products (AGEs), which are nonenzymatically glycated
Bovine erythrocyte superoxide dismutase: diazo coupling, subunit interactions, and electrophoretic variants.
D P Malinowski et al.
Biochemistry, 18(1), 237-244 (1979-01-09)
Y Ohara et al.
The Journal of clinical investigation, 91(6), 2546-2551 (1993-06-01)
Indirect evidence suggests accelerated degradation of endothelium-derived nitric oxide (ENDO) by superoxide anion (O2-) in hypercholesterolemic vessels (HV). To directly measure O2- production by normal vessels (NV) and HV, we used an assay for O2- based on the chemiluminescence (CL)
Luciana Cacciottola et al.
Fertility and sterility, 110(3), 534-544 (2018-07-02)
To characterize oxidative stress and metabolic activity in xenografted human ovarian tissue using microdialysis. Prospective experimental study. Gynecology research unit at a university hospital. Cryopreserved ovarian cortex from five women 27-35 years of age. Frozen-thawed human ovarian tissue fragments were xenografted
Naoya Ichimaru et al.
Biochemistry, 47(40), 10816-10826 (2008-09-11)
The mode of action of Deltalac-acetogenins, strong inhibitors of bovine heart mitochondrial complex I, is different from that of traditional inhibitors such as rotenone and piericidin A [Murai, M., et al. (2007) Biochemistry 46 , 6409-6416]. As further exploration of
Protocoles
Enzymatic Assay of Superoxide Dismutase
Separation of Superoxide dismutase
Chromatograms
application for HPLCNotre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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