P3738

Protein Kinase G Iβ human

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution

• Synonyme(s) : cyclic-Guanosine Monophosphate-dependent Protein Kinase 1β human
• Numéro de classification (Commission des enzymes): 2.7.11.12 (BRENDA, IUBMB)
• Numéro MDL: MFCD03458230
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in baculovirus infected Sf9 cells
• Niveau de qualité: 200
• Pureté: ≥95% (SDS-PAGE)
• Forme: buffered aqueous glycerol solution
• Activité spécifique: ≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))
• Poids mol.: 76 kDa (monomer)
• Numéro d'accès UniProt: Q13976
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... PRKG1(5592)

Description

Application

Protein Kinase G is a serine/threonine-specific protein kinase that is activated by cGMP. Protein Kinase G Iβ is used to induce apoptosis and inhibit cell proliferation.

Actions biochimiques/physiologiques

Protein Kinase G Iβ induces apoptosis in certain cell lines such as human breast cancer cell lines MCF-7 and MDA-MB-468. It inhibits cell proliferation and induces apoptosis in colon cancer cell lines.

Définition de l'unité

One unit will phosphorylate 1 micromole of VASPtide(RRKVSKQE) substrate per minute in 10 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM DTE and 0.2 mM EDTA.

Forme physique

Solution in 20 mM Tris buffer, pH 7.4, 1 mM EDTA, 1 mM β-mercaptoethanol, 100 mM NaCl, 10 U/ml Trasylol, and 50% glycerol.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable