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L3540

Supelco

Lowry Reagent

Synonyme(s) :

Copper(II) tartrate Mix

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Niveau de qualité

100

Description générale

The Lowry method has been for decades the procedure of choice for quantitation of soluble proteins due to its sensitivity, simplicity, and precision. Peterson′s modification of the micro Lowry method is simple, rapid, objective and more generally applicable. In this procedure sodium dodecyl sulfate (SDS) is mixed with Lowry reagent to enable the dissolution of relatively insoluble lipoproteins.

Application

For protein determination. Utilizes SDS to facilitate dissolution of relatively insoluble lipoporoteins.
Lowry Reagent has been used to quantify the protein content in extracellular polymeric substance (EPS). It has also been used to quantify total protein and mitochondrial protein in Drosophila melanogaster.

Autres remarques

Dry powder mixture containing Lowry reagent with sodium dodecyl sulfate.

Reconstitution

Reconstitute to 40 mL with deionized water.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

FlameCorrosionExclamation mark
GHS02,GHS05,GHS07

Mention d'avertissement

Danger

Mentions de danger

H228,H302,H314,H412

Classification des risques

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Corr. 1B

Code de la classe de stockage

4.1B - Flammable solid hazardous materials

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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A simplification of the protein assay method of Lowry et al. which is more generally applicable
Peterson GL
Analytical Biochemistry, 83(2), 346-356 (1977)
Environmental and genetic perturbations reveal different networks of metabolic regulation
Greenberg A, et al.
Molecular Systems Biology, 7(1), 563-563 (2011)
Raffinose, a plant galactoside, inhibits Pseudomonas aeruginosa biofilm formation via binding to LecA and decreasing cellular cyclic diguanylate levels
Kim HS, et al.
Scientific reports, 6(1), 25318-25318 (2016)
Ginger extract inhibits biofilm formation by Pseudomonas aeruginosa PA14
Kim HS and Park HD
PLoS ONE, 8(9), e76106-e76106 (2013)
So-Young Ham et al.
Chemosphere, 268, 128802-128802 (2020-11-10)
Biofouling is a major operational problem in the reverse osmosis (RO) process, affecting the membrane performance. Although sodium hypochlorite (NaOCl) is used to chemically clean the biofouled membranes, high concentrations of NaOCl cause morphological and chemical damage to the RO
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Protocoles

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Contenu apparenté

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

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