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GE28-4082-61

HiTrap® MabSelect™ Xtra

Cytiva 28-4082-61, pack of 5 × 5 mL

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

ligand

recombinant protein A (E. coli)

Conditionnement

pack of 5 × 5 mL

Fabricant/nom de marque

Cytiva 28-4082-61

Conditions de stockage

(20% Ehtanol)
 (20% Ethanol)

Paramètres

42 psi

Taille du lit

16 mm × 25 mm

Volume du lit

5 mL

D.I. de la colonne

16 mm

Matrice

highly cross-linked agarose

Taille moy. des particules

75 (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-12

working range

3-10

Capacité

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

Adéquation

suitable for bioprocess medium

Température de stockage

2-8°C

Description générale

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap® MabSelect™ Xtra columns are prepacked with MabSelect™ Xtra, a BioProcess medium for capture of monoclonal antbodies from large sample volumes.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Application

The increased capacity of the medium is well suited for purification of Mabs from high-level expression feedstock.

Caractéristiques et avantages

  • Increased dynamic binding capacity with smaller particle size and greater porosity than MabSelect™ medium.
  • MabSelect™ Xtra uses the same recombinant Protein A ligand as MabSelect™
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Prepacked HiTrap® columns for process development, screening of purification conditions, and small-scale purification of Mabs.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Column Packing and Preparation for Affinity Chromatography of Antibodies

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This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

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