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GE17-5110-01

HiTrap® IGM purification

Cytiva 17-5110-01, pack of 5 × 1 mL

Synonyme(s) :

IGM Purification Column

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

ligand

2-mercaptopyridine

Conditionnement

pack of 5 × 1 mL

Fabricant/nom de marque

Cytiva 17-5110-01

Conditions de stockage

(20% Ethanol)

Paramètres

<4 mL/min flow rate
42 psi

Taille du lit

7 mm × 25 mm

Volume du lit

1 mL

D.I. de la colonne

16 mm

Matrice

highly cross-linked 6% agarose

Taille moy. des particules

34 μm

cleaning

2-13

working range

3-11

Température de stockage

2-8°C

Description générale

HiTrap® IgM Purification HP is a 1 mL column prepacked with Sepharose™ High Performancefor fast and efficient purification of monoclonal IgM.

Application

HiTrap® IgM Purification HP is prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Caractéristiques et avantages

  • Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
  • Prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance.
  • Recovery of IgM is high with excellent activity retention.
  • The top and bottom frits are manufactured from porous polyethylene
  • Columns are delivered with a stopper on the inlet and a snap-off end on the outle

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP
Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocoles

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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