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Key Documents

GE17-0440-03

Con-A Sepharose™ 4B

Cytiva 17-0440-03, pack of 5 mL

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

concanavalin A (con A)

Durée de conservation

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Conditionnement

pack of 5 mL

Fabricant/nom de marque

Cytiva 17-0440-03

Conditions de stockage

(0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)

Matrice

4% agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning in place

4-9

working range

4-6

Température de stockage

2-8°C

Catégories apparentées

Description générale

Con A Sepharose™ 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose.

Application

Con A Sepharose™ is Concanavalin A coupled to Sepharose™ 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloProtein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose™ is routinely used for separation and purification of glycoProteins, polysaccharides and glycolipids

Caractéristiques et avantages

  • Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Group-specific adsorbent for molecules containing sugars.
  • Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes
  • Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes
  • Con A coupled to Sepharose™ 4B via cyanogen bromide activation

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocoles

Purification or Removal of Glycoproteins and Polysaccharides

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from GE Healthcare.

Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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