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G0924

Millipore

Glutathione High Capacity Magnetic Agarose Beads

(1:1 suspension in a 30% ethanol solution)

Synonyme(s) :

Magnetic agarose beads

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About This Item

Numéro MDL:
MFCD00131200
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Conjugué

magnetic beads

Niveau de qualité

200

Forme

(1:1 suspension in a 30% ethanol solution)

Classe(s) chimique(s) de l'analyte

proteins (GST)

Technique(s)

immunoprecipitation (IP): suitable
protein purification: suitable

Matrice

4% beaded magnetic agarose

Espaceur de matrice

12 atoms

Capacité

≥15 μmol/mL, agarose binding capacity

Conditions d'expédition

wet ice

Température de stockage

2-8°C

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Description générale

Glutathione Magnetic Agarose Beads consist of a paramagnetic 4% beaded agarose. Glutathione is covalently attached through the sulfur to epoxy-activated agarose resulting in a 12 atom (10 carbon) spacer.

The Glutathione Magnetic Agarose Beads are useful for affinity capture, molecular pull-down, or immunoprecipitation (IP) of proteins containing glutathione binding sequences, such as native glutathione S-transferase (GST), glutathione peroxidase, and glyoxalase I, or GST-tagged recombinant fusion proteins from cell lysates or other biochemical solutions while exhibiting low non-specific binding of other proteins. Recombinant GST-tagged proteins, bound to the affinity resin are separated with the use of a magnet. The magnetic properties allow for very rapid processing, as well as aid in manipulations, such as repetitive washings and recovery of the desired protein. This leads to faster recovery, experimental reproducibility, and more accurate quantitation of the proteins of interest.

The matrix of the magnetic beads is a 4% beaded agarose with an average diameter of 50 μ and a diameter range of 20-75 μ. Paramagnetic iron is impregnated within the beads.

Application

Useful for affinity capture, molecular pull-down, or immunoprecipitation (IP) of proteins containing glutathione binding sequences, such as recombinant GST-tagged fusion proteins or native glutathione S-transferase, glutathione peroxidase and glyoxalase I from cell lysates and other biological samples. Can be used for purification in single tubes or for high throughput in 96-well multiwell plates. The magnetic properties of the beads aid in manipulations, such as repetitive washings and recovery of the protein. This leads to greater experimental reproducibility and more accurate quantitation of the proteins of interest.

Forme physique

1:1 suspension in a 30% ethanol solution

Pictogrammes

Flame
GHS02

Mention d'avertissement

Danger

Mentions de danger

H225

Classification des risques

Flam. Liq. 2

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 2

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Xueran Chen et al.
Theranostics, 10(3), 998-1015 (2020-01-16)
Rationale: Glioblastoma multiforme (GBM) almost invariably gain invasive phenotype with limited therapeutic strategy and ill-defined mechanism. By studying the aberrant expression landscape of gliomas, we find significant up-regulation of p-MAPK level in GBM and a potent independent prognostic marker for
Reinier F Prosée et al.
PLoS biology, 19(7), e3000968-e3000968 (2021-07-07)
Centromere protein A (CENP-A) is a histone H3 variant that defines centromeric chromatin and is essential for centromere function. In most eukaryotes, CENP-A-containing chromatin is epigenetically maintained, and centromere identity is inherited from one cell cycle to the next. In
April N Ilacqua et al.
BMC cell biology, 19(1), 13-13 (2018-07-18)
Copines are calcium-dependent phospholipid-binding proteins found in many eukaryotic organisms and are thought to be involved in signaling pathways that regulate a wide variety of cellular processes. Copines are characterized by having two C2 domains at the N-terminus accompanied by
Juliana S Capitanio et al.
eLife, 6 (2017-02-22)
Beyond their role at nuclear pore complexes, some nucleoporins function in the nucleoplasm. One such nucleoporin, Nup98, binds chromatin and regulates gene expression. To gain insight into how Nup98 contributes to this process, we focused on identifying novel binding partners
Maria Yurchenko et al.
The Journal of cell biology, 217(4), 1411-1429 (2018-02-15)
Signaling lymphocytic activation molecule family 1 (SLAMF1) is an Ig-like receptor and a costimulatory molecule that initiates signal transduction networks in a variety of immune cells. In this study, we report that SLAMF1 is required for Toll-like receptor 4 (TLR4)-mediated
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Protein Pull-Down

Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

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