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E7885

Sigma-Aldrich

Elastase from porcine pancreas

lyophilized powder, suitable for cell culture

Synonyme(s) :

Elastase from hog pancreas, Pancreatopeptidase E

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About This Item

Numéro CAS:
39445-21-1
Numéro de classification (Commission des enzymes):
3.4.21.36 (BRENDA, IUBMB)
Numéro CE :
254-453-6
Numéro MDL:
MFCD00130998
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.75

Source biologique

Porcine

Niveau de qualité

200

Forme

lyophilized powder

Activité spécifique

≥4 units/mg protein

Poids mol.

25.9 kDa

Conditionnement

pkg of 1 μmol

Technique(s)

cell culture | mammalian: suitable

pH

8

Adéquation

suitable for

Activité étrangère

Trypsin ≤50 BAEE units/mg protein

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.

Application

Elastase from porcine pancreas is used for the following applications:
  • Cell culture techniques
  • ELISA techniques
  • Used in assay for elastase inhibitory activity

Actions biochimiques/physiologiques

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

Conditionnement

Package size based on protein content

Définition de l'unité

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Application

Réf. du produit
Description
Tarif

Inhibiteur

Réf. du produit
Description
Tarif

Substrat

Réf. du produit
Description
Tarif

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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P Hamet et al.
The Journal of biological chemistry, 264(21), 12364-12369 (1989-07-25)
The induction of cyclic GMP formation in target tissues, i.e. vascular smooth muscle and endothelial cells, by atrial natriuretic factor is followed by its egression into plasma and urine. Since the extracellular appearance of this cyclic nucleotide is used as
F Seibold et al.
Gut, 32(10), 1192-1197 (1991-10-01)
The presence of antibodies against pancreatic juice (PAB) in patients with Crohn's disease has recently been reported. In our study sera from 273 patients with inflammatory bowel disease (222 with Crohn's disease, 51 with ulcerative colitis) have been examined for
Yoshiyuki Okumura et al.
Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology, 48(1), 13-18 (2007-02-09)
The amino acid sequence of elastase inhibitor, AFLEI, isolated from Aspergillus flavus was determined by the Edman sequencing procedure of peptides derived from digests utilizing clostripain. A molecular weight of 7,525.8 was observed by TOF-MS. AFLEI contained 68 amino acid
Adrien Lupieri et al.
Cardiovascular research, 116(2), 438-449 (2019-05-21)
Defects in efficient endothelial healing have been associated with complication of atherosclerosis such as post-angioplasty neoatherosclerosis and plaque erosion leading to thrombus formation. However, current preventive strategies do not consider re-endothelialization in their design. Here, we investigate mechanisms linking immune
Hao Li et al.
Nature communications, 11(1), 3218-3218 (2020-06-27)
Unruptured intracranial aneurysm (UIA) is a life-threatening cerebrovascular condition. Whether changes in gut microbial composition participate in the development of UIAs remains largely unknown. We perform a case-control metagenome-wide association study in two cohorts of Chinese UIA patients and control
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Protocoles

Enzymatic Assay of Elastase (EC 3.4.21.36)

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

Chromatograms

application for HPLC

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