C1682

Acetylcholinesterase human

recombinant, expressed in HEK 293 cells, lyophilized powder, ≥1,000 units/mg protein (Lowry)

• Synonyme(s) : AChE, Acetylcholine acetylhydrolase
• Numéro CAS: 9000-81-1
• Numéro de classification (Commission des enzymes): 3.1.1.7 (BRENDA, IUBMB)
• Numéro CE : 232-559-3
• Numéro MDL: MFCD00081268
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.32

Propriétés

• Produit recombinant: expressed in HEK 293 cells
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: ≥1,000 units/mg protein (Lowry)
• Poids mol.: 64.6 kDa
• Numéro d'accès UniProt: P22303
• Température de stockage: −20°C
• Informations sur le gène: human ... ACHE(43)

Description

Description générale

Acetylcholinesterase (AChE) is a serine hydrolase, which belongs to the carboxyl esterase family of enzymes. AChE is localised at neuromuscular junctions and cholinergic brain synapses.

Application

Acetylcholinesterase human has been used:

• as a standard protein to measure protein levels acetylcholinesterase AChE-R and AChE-S
• to stimulate human fibroblasts
• to study its in vitro catalytic activity and to determine the effects of metals, H₂O₂ and OH radicals on the activity

Actions biochimiques/physiologiques

Acetylcholinesterase (AChE) is regarded as a biomarker in neurotoxicity. It is a modulator of nitric oxide signal transduction pathway and marker of membrane integrity and aging. AChE, hydrolyzes choline esters. It terminates the impulse transmission at cholinergic synapses. AChE does this by rapid hydrolysis of the neurotransmitter acetylcholine (ACh) to acetate and choline. AChE inhibitors prevent the cholinesterase enzyme from breaking down ACh and increases the level and duration of the neurotransmitter action.

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H₂O to choline + acetic acid.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of acetylthiocholine to thiocholine and acetate per minute at pH 8.0 at 37 °C.

Forme physique

This product is supplied as a lyophilized powder. Lyophilized from 0.22 μm filtered solution in 50mM phosphate buffer pH8.

Remarque sur l'analyse

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable