C0663

Acetylcholinesterase from human erythrocytes

buffered aqueous solution, ≥500 units/mg protein (BCA)

• Synonyme(s) : AChE, Acetylcholine acetylhydrolase
• Numéro CAS: 9000-81-1
• Numéro de classification (Commission des enzymes): 3.1.1.7 (BRENDA, IUBMB)
• Numéro CE : 232-559-3
• Numéro MDL: MFCD00081268
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: human erythrocytes
• Niveau de qualité: 200
• Forme: buffered aqueous solution
• Activité spécifique: ≥500 units/mg protein (BCA)
• Poids mol.: ~80 kDa
• Numéro d'accès UniProt: P22303
• Température de stockage: 2-8°C
• Informations sur le gène: human ... ACHE(43)

Description

Description générale

Acetylcholinesterase (AChE) belongs to the carboxyl esterase family of enzymes. The erythrocyte AChE is membrane bound. AChE is mapped to human chromosome 7q22.1. It is enriched in aged erythrocytes.

Predominantly exists as a tetrameric glycoprotein composed of disulfide-linked homodimers with a monomer MW of ~80 kDa.

Application

Acetylcholinesterase (AChE) from Sigma has been used in the structure-activity study of phosphoramido acid esters as inhibitors of AChE.

Acetylcholinesterase from human erythrocytes has been used in:

• cholinesterase inhibition assay for screening 4-aminoquinoline based compounds
• AChE activity assays to test the effect of positive allosteric modulators (PAMs)
• organophosphorus compounds based inhibition assay

Actions biochimiques/physiologiques

Acetylcholinesterase (AChE) is regarded as a biomarker in neurotoxicity. It is a modulator of nitric oxide signal transduction pathway and marker of membrane integrity and aging. The levels of erythrocyte (RBC) AChE are affected on pesticide exposure and in hemolytic anemia. RBC AChE is a marker in Hirschsprung′s disease and inflammation.

Acetylcholinesterase is the major in vivo degradative enzyme for acetylcholine. It converts acetylcholine and water to choline and acetic acid. Cholinesterases are inhibited by the natural carbamate alkaloid, eserine or physostigmine.

In blood there are two cholinesterases present: The erythrocyte associated enzyme, which is a true cholinesterase or acetylcholinesterase [(AChE) - E.C. 3.1.1.7], the serum associated enzyme, which is Pseudocholinesterase or Butyrylcholinesterase [(BuChE) - EC 3.1.1.8].
AChE is an ectoenzyme, anchored to the erythrocyte membrane via a GPI moiety.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of acetylthiocholine iodide per min at pH 7.4 at 37 °C.

Forme physique

Solution in 20 mM HEPES, pH 8.0, containing 0.1% TRITON® X-100

Notes préparatoires

The enzyme is the amphiphilic form extracted together with its GPI anchor with the aid of TRITON X-100 and purified by affinity chromatography.

Remarque sur l'analyse

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)