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11442074001

Roche

BM-Purple

Roche, pkg of 100 mL, solution

Synonyme(s) :

chromogenic substrate

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About This Item

Code UNSPSC :
12352204

Forme

solution

Niveau de qualité

100

Conditionnement

pkg of 100 mL

Fabricant/nom de marque

Roche

Couleur

dark purple

Solubilité

H2O: insoluble

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.

Appearance prior to use: light yellow color;
After color development: dark purple

Application

  • Colorimetric AP substrate for: Immunohistocytochemistry
  • Western blot
  • Southern blot
  • Northern blot
  • Colony and plaque hybridization
  • In situ hybridization

Composants

Ready-to-use solution.

Notes préparatoires

Working concentration: Best results have been obtained using 200 mU POD and casein as blocking reagent.
Working solution: Solubility in water: insoluble.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

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Christoph Kessle
Nonradioactive Analysis of Biomolecules null
Valentina Garcia-Lee et al.
The International journal of developmental biology, 65(4-5-6), 395-401 (2020-09-16)
The cell differentiation of the musculoskeletal system is highly coordinated during limb development. In the distal-most region of the limb, WNT and FGF released from the apical ectodermal ridge maintain mesenchymal cells in the undifferentiated stage. Once the cells stop
Ning Pan et al.
PloS one, 7(1), e30358-e30358 (2012-01-27)
Atonal homolog1 (Atoh1) is a bHLH transcription factor essential for inner ear hair cell differentiation. Targeted expression of Atoh1 at various stages in development can result in hair cell differentiation in the ear. However, the level and duration of Atoh1
Yoshihiro Komatsu et al.
Methods in molecular biology (Clifton, N.J.), 1092, 1-15 (2013-12-10)
In situ hybridization is a powerful method for detecting endogenous mRNA sequences in morphologically preserved samples. We provide in situ hybridization methods, which are specifically optimized for mouse embryonic samples as whole mounts and section tissues. Additionally, β-Galactosidase (β-gal) is

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