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11383221001

Roche

BCIP

solution, >95% (HPLC), pkg of 3 mL (150 mg)

Synonyme(s) :

4-toluidine salt

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About This Item

Code UNSPSC :
12352204

Description

4-toluidine salt

Niveau de qualité

Pureté

>95% (HPLC)

Forme

solution

Conditionnement

pkg of 3 mL (150 mg)

Fabricant/nom de marque

Roche

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

BCIP serves as a substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Application

Use BCIP in alkaline phosphatase detection:
  • Southern blot
  • Northern blot
  • Western blot
  • Colony and plaque hybridization
  • In situ hybridization

Qualité

Function test: incubation with AP

Principe

Use of BCIP as substrate for alkaline phosphatase in combination with nitro blue tetrazolium chloride (NBT) as electron acceptor: Substrates and reaction products of alkaline phosphatase catalyzed color reaction with NBT/BCIP.

Forme physique

Solution (50 mg/ml) in dimethylformamide (DMF)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Pictogrammes

FlameExclamation markHealth hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Acute Tox. 4 - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 Dermal - Repr. 1B

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 2


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Gregor Eichele et al.
Methods (San Diego, Calif.), 53(4), 417-423 (2010-12-28)
Genome-scale sequencing projects, high-throughput RNAi screens, systematic gene targeting, and system-biology-based network predictions all depend on a validation of biological significance in order to understand the relevance of a particular finding. Such validation, for the most part, rests on low-throughput
Nicoletta I Petridou et al.
Cell, 184(7), 1914-1928 (2021-03-18)
Embryo morphogenesis is impacted by dynamic changes in tissue material properties, which have been proposed to occur via processes akin to phase transitions (PTs). Here, we show that rigidity percolation provides a simple and robust theoretical framework to predict material/structural
Ewa Pocheć et al.
Acta biochimica Polonica, 62(2), 323-328 (2015-06-23)
Although both uveal (UM) and cutaneous (CM) melanoma cells derive from the transformed melanocytes, their biology varies significantly in several aspects. Malignant transformation is frequently associated with alternations in cell glycosylation, in particular those concerning branched complex-type N-glycans. These changes
Zoe Crane-Smith et al.
Frontiers in cell and developmental biology, 9, 595744-595744 (2021-04-20)
Enhancers that are conserved deep in evolutionary time regulate characteristics held in common across taxonomic classes. Here, deletion of the highly conserved Shh enhancer SBE2 (Shh brain enhancer 2) in mouse markedly reduced Shh expression within the embryonic brain specifically
Valentina Vazzola et al.
FEBS letters, 581(4), 667-672 (2007-01-30)
Frataxin is present in mitochondria of all eukaryotes as well as in the cytoplasm of bacteria. In humans, reduced expression of frataxin is associated with Friedreich's ataxia, a recessive inherited neurodegenerative and cardiac disorder leading to reduced life expectancy. Experimental

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