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11426338910

Roche

Anti-Fluorescein-AP, Fab fragments

from sheep

Synonyme(s) :

antibody

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About This Item

Code UNSPSC :
12352200

Source biologique

sheep

Niveau de qualité

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

solution

Conditionnement

pkg of 150 U (200 μl)

Fabricant/nom de marque

Roche

Isotype

IgG

Température de stockage

2-8°C

Description générale

Fluorescein is synthesized from m-dialkylaminophenols or resorcinols condensed with phthalic anhydrides under harsh conditions. Fab fragments bind to G proteins.
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. (provided by Ref Seq)

Spécificité

The polyclonal antibody reacts with free and bound fluorescein.

Application

Use Anti-Fluorescein-AP, Fab fragments for the detection of fluorescein-labeled compounds using:
  • Dot blot
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Southern blot
  • Western blot
  • Electrochemical biosensing technique for the detection of 16S rRNA

Actions biochimiques/physiologiques

Anti-Fluorescein-AP, Fab fragments has been used in oligonucleotide capture, denaturation and detection (CDD).

Caractéristiques et avantages

Contents
Solution, stabilized

Notes préparatoires

  • Working concentration: The following concentrations depend on application and substrate and should be taken as a guideline. Dot blot: 150mU/ml
  • ELISA: 150 to 300mU/ml
  • Immunohistocytochemistry: 250 to 500mU/ml
  • In situ hybridization: 1.5 to 7.5U/ml
  • Southern blot: 150mU/ml
  • Western blot: 250 to 500 mU/ml

Working solution: 100mM Tris-HCl, 150mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

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Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

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PLoS ONE (2016)
Nadia A Fernández-Santos et al.
PLoS neglected tropical diseases, 14(1), e0008008-e0008008 (2020-01-31)
All formerly endemic communities of the Southern Chiapas focus of onchocerciasis in Mexico were treated with ivermectin until parasite transmission was eliminated by 2015. Transmission of onchocerciasis did not resume during a period of three years (2012-2014) following the final
Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores
Minta A, et al.
The Journal of Biological Chemistry, 264(14), 8171-8178 (1989)
Mohammad Goudarzi et al.
Developmental cell, 43(5), 577-587 (2017-11-28)
Cell migration is essential for morphogenesis, organ formation, and homeostasis, with relevance for clinical conditions. The migration of primordial germ cells (PGCs) is a useful model for studying this process in the context of the developing embryo. Zebrafish PGC migration

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