Direkt zum Inhalt
Merck

HPLC Analysis of Organic Acids on Ascentis® Express RP-Amide

HPLC Analysis of Organic Acids on Ascentis® Express RP-Amide application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

Ascentis® Express RP-Amid, 2,7 μm HPLC-Säule

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

Standard

Produkt-Nr.
Beschreibung
Preisangaben

Zitronensäure

99%

Fumarsäure

≥99.0% (T)

L-(+)-Tartarsäure

≥99.5%

Acrylsäure

anhydrous, contains 200 ppm MEHQ as inhibitor, 99%

Milchsäure

Pharmaceutical Secondary Standard; Certified Reference Material

CONDITIONS

column

Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)

mobile phase

[A] 0.1% (v/v) TFA in [B] water

flow rate

0.3 mL/min

column temp.

35 °C

detector

UV, 210 nm

injection

1 μL

sample

0.2 - 4 g/L in mobile phase

Beschreibung

Hinweis zur Analyse

This application demonstrates the suitability of Ascentis Express RP-Amide for the analysis of organic acids under 100% aqueous conditions.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany