X0879

Anti-XPB in Kaninchen hergestellte Antikörper

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-BTF2, Anti-ERCC3, Anti-Excision repair cross-complementing rodent repair deficiency, complementation group 3
• MDL-Nummer: MFCD02263308
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 90 kDa
• Speziesreaktivität: human, rat, mouse
• Konzentration: ~1 mg/mL
• Methode(n): indirect immunofluorescence: 10-20 μg/mL using HeLa cells fixed with paraformaldehyde-Triton; western blot: 1-2 μg/mL using HepG2 cell lysates
• UniProt-Hinterlegungsnummer: P19447
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ERCC3(2071) ; mouse ... Ercc3(13872) ; rat ... Ercc3(291703)

Beschreibung

Allgemeine Beschreibung

XPB (Xeroderma pigmentosum complementation group B) is located on the human chromosome at 2q14.3. TFIIH (General transcription and DNA repair factor IIH helicase) consists of nine protein subunits, XPB (ERCC3) being one of them. It is an ATP-dependent DNA helicase of 89 kDa. The XPB is localized in the neurons.

Immunogen

synthetic peptide corresponding to amino acids 765-782 of human XPB, also known as ERCC3, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide differs from the mouse and rat sequences in one and three amino acids respectively.

Anwendung

Anti-XPB antibody has been used in immunoblotting andimmunofluorescence.

Biochem./physiol. Wirkung

XPB (General transcription and DNA repair factor IIH helicase subunit XPB) is involved in DNA repair and transcription. Phosphorylation on Ser⁷⁵¹ of XPB inhibits TFIIH repair activity, leaving its transcription function intact. Mutations in the gene encoding XPB causes Xeroderma Pigmentosum, Cockayne′s syndrome, and trichothiodystrophy.

Physikalische Form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)