T7575
Trypsin, Singles, Proteomik-Qualität
Synonym(e):
Proteomics grade, Trypsin, TPCK treated
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About This Item
UNSPSC-Code:
12352204
NACRES:
NA.56
Empfohlene Produkte
Qualität
Proteomics Grade
Qualitätsniveau
Form
ready-to-use solution
Spezifische Aktivität
≥10,000 BAEE units/mg protein
Mol-Gew.
average mol wt 23.29 kDa
Optimaler pH-Wert
~8.0
Lagertemp.
2-8°C
Allgemeine Beschreibung
Experience all of the advantages of Proteomics Grade Trypsin in a convenient, single-use 1 μg package.
Trypsin Singles, Proteomics Grade exhibit excellent proteolytic efficiency, generating more tryptic peptides leading to greater sequence coverage of your peptide of interest. Mass spectra are significantly simplified due to the reduced number of interfering autolytic peaks and their ambiguous adducts. Proteomics Grade Trypsin has been extensively purified from porcine pancreas to enable accurate and precise cleavage on the carboxylic side of Arg and Lys residues. The enzyme has been exhaustively processed by reductive methylation to minimize autolysis and chymotryptic activity has been quenched by TPCK treatment. Further purification steps including affinity chromatography and drying from dilute acid produce a highly purified, high specific activity trypsin purposely suited for the demanding criteria of proteomics research, and designed to function in either solution or gel-based digests.
Trypsin Singles, Proteomics Grade exhibit excellent proteolytic efficiency, generating more tryptic peptides leading to greater sequence coverage of your peptide of interest. Mass spectra are significantly simplified due to the reduced number of interfering autolytic peaks and their ambiguous adducts. Proteomics Grade Trypsin has been extensively purified from porcine pancreas to enable accurate and precise cleavage on the carboxylic side of Arg and Lys residues. The enzyme has been exhaustively processed by reductive methylation to minimize autolysis and chymotryptic activity has been quenched by TPCK treatment. Further purification steps including affinity chromatography and drying from dilute acid produce a highly purified, high specific activity trypsin purposely suited for the demanding criteria of proteomics research, and designed to function in either solution or gel-based digests.
Anwendung
Trypsin Singles, Proteomics Grade have been used for protein digestion to analyse generated peptides via MALDI-TOF MS (matrix assisted laser desorption ionization-time of flight mass spectrometry). It has been used for the digestion of proteins to analyse generated peptides via LC-MS (liquid chromatography–mass spectrometry)/MS.
Leistungsmerkmale und Vorteile
- Bequemer Einwegartikel, 1 μg Größe
- Eliminiert wiederholtes Pipettieren
- eduktiv methyliert, um autolytische Aktivität zu minimieren
- TPCK behandelt, um chymotryptische Aktivität zu dämpfen
- Extensiv gereinigt
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Produkt-Nr.
Beschreibung
Preisangaben
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Zielorgane
Respiratory system
Lagerklassenschlüssel
3 - Flammable liquids
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