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Merck

T2928

Sigma-Aldrich

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse

clone TH-16, ascites fluid

Synonym(e):

Anti-DYT14, Anti-DYT5b, Anti-TYH

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

TH-16, monoclonal

Enthält

15 mM sodium azide

Speziesreaktivität

rat, sheep, human, monkey, bovine, rabbit, guinea pig

Methode(n)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable using native or SDS-denatured TH.
microarray: suitable
western blot: 1:8,000 using extracts of cultured rat adrenal pheochromocytoma (PC12) cells.

Isotyp

IgG1

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... TH(7054)
rat ... Th(25085)

Allgemeine Beschreibung

Monoclonal Anti-Tyrosine Hydroxylase (mouse IgG1 isotype) is derived from the TH-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Tyrosine hydroxylase (TH) exists in four isoforms in humans. They are are TH-1 (M.W. 55,600), TH-2 (4 additional amino acids; M.W. 56,000), TH-3, (27 additional amino acids; M.W. 58,100), and TH-4 (4 plus 27 additional amino acids; 58,500). Only humans express all four isoforms; all other anthropoids express only the TH-1 and TH-2 isoforms.
Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O2 as cosubstrates, and Fe2+ as a cofactor.

Spezifität

The antibody recognizes an epitope present in the N-terminal region (between aa 40-152) of both rodent and human tryosine hydroxylase. TH-16 detects both the intact subunits and the 59/57 kDa doublet and an array of decreasing molecular weight TH forms resulting from severe proteolysis.

Immunogen

rat tyrosine hydroxylase (TH).

Anwendung

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse has also been used in
  • western blotting
  • immunofluorescence labelling
  • immunohistochemical staining
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • immunoprecipitation
  • immunocytochemistry

Monoclonal Anti-tyrosine hydroxylase antibody produced in mouse has been used in immunohistochemistry and double immunofluorescence labeling.

Biochem./physiol. Wirkung

Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson′s disease (PD).

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Influence of aging on the dopaminergic neurons in the substantia nigra pars compacta of rats
Gao J, et al.
Current Aging Science, 4(1), 19-24 (2011)
Selective cholinergic depletion of pedunculopontine tegmental nucleus aggravates freezing of gait in parkinsonian rats
Xiao H, et al.
Neuroscience Letters, 659, 92-98 (2017)
Evelyn H Schlenker et al.
Brain research, 1401, 40-51 (2011-06-15)
Hypothyroidism can lead to depressed breathing. We determined if propylthiouracil (PTU)-induced hypothyroidismin hamsters (HH) altered dopamine D1 receptor expression, D1 receptor-modulated ventilation, and ventilatory chemoreflex activation by hypoxia or hypercapnia. Hypothyroidism was induced by administering 0.04% PTU in drinking water
Monica S Montesinos et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(13), 3350-3358 (2008-03-28)
Chromogranins (Cgs) are the major soluble proteins of dense-core secretory vesicles. Chromaffin cells from Chga null mice [chromogranin A knock-out (CgA-KO)] exhibited approximately 30% reduction in the content and in the release of catecholamines compared with wild type. This was
The astrocytic response to the dopaminergic denervation of the striatum.
Morales I, et al.
Journal of Neurochemistry, 139(1), 81-95 (2016)

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