T1428
apo-Transferrin bovine
BioReagent, suitable for cell culture, ≥98%
Synonym(e):
Human serotransferrin
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About This Item
Empfohlene Produkte
Biologische Quelle
bovine
Produktlinie
BioReagent
Assay
≥98%
Form
powder
Methode(n)
cell culture | mammalian: suitable
Verunreinigungen
iron, essentially free
Löslichkeit
H2O: 10 mg/mL
UniProt-Hinterlegungsnummer
Lagertemp.
2-8°C
Angaben zum Gen
bovine ... TF(280705)
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Allgemeine Beschreibung
apo-Transferrin is an iron-free form of transferrin that is present in circulation.
Anwendung
All cells require iron for the proper uptake of oxygen from their environment. Transferrin, a protein isolated from serum, causes the uptake and transport of iron from culture medium to the cells. This allows proper oxygen uptake and also stimulates growth-related enzyme activity. Iron-saturated transferrin (holo transferrin) is continually recycled after releasing iron and becomes iron-deficient transferrin (apo-transferrin). Choosing which form to use depends upon the culture conditions. In media such as Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12 (DMEM/F12) that contain high levels of iron salt, using apo-transferrin is preferred.
apo-Transferrin bovine has been used as a component of:
- basal medium for culturing bovine mammary epithelial cells
- minimum essential medium α (MEM-α) for spermatogonial stem cells (SSCs) culture
- induction medium for mesenchymal stem cell culture
Biochem./physiol. Wirkung
apo-Transferrin (apoTf) aids in preventing iron accumulation in tissues, especially in non-transfusion dependent β-thalassemia.
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Monique P Gelderman et al.
Haematologica, 100(5), 611-622 (2015-01-27)
Intermediate beta-thalassemia has a broad spectrum of sequelae and affected subjects may require occasional blood transfusions over their lifetime to correct anemia. Iron overload in intermediate beta-thalassemia results from a paradoxical intestinal absorption, iron release from macrophages and hepatocytes, and
Keita Abe et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 63(1-2), 154-156 (2008-04-05)
We have investigated the mechanism of manganese ion uptake by apo-transferrin using a capillary electrophoresis method, and obtained clear evidence that oxidation state +3 and the binuclear unit of a manganese chelate are essential factors for the facile uptake by
Jia Wang et al.
Stem cell research & therapy, 10(1), 227-227 (2019-07-31)
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Journal of animal science and biotechnology, 7, 44-44 (2016-08-06)
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