T5391

apo-Transferrin human

γ-irradiated, powder, BioXtra, suitable for cell culture, ≥98%

• Synonym(e): Humanes Transferrin, Siderophilin
• CAS-Nummer: 11096-37-0
• EG-Nummer: 234-318-8
• MDL-Nummer: MFCD00130536
• UNSPSC-Code: 12352202
• NACRES: NA.75

Eigenschaften

• Qualitätsniveau: 200
• Sterilität: γ-irradiated
• Produktlinie: BioXtra
• Assay: ≥98%
• Form: powder
• Mol-Gew.: 76-81 kDa
• Konzentration: ~25 mM
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: HIV and HBsAg, source material tested negative; iron, essentially free
• Löslichkeit: H₂O: 50 mg/mL
• UniProt-Hinterlegungsnummer: P02787
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... TF(7018)

Beschreibung

Allgemeine Beschreibung

apo-Transferrin (aTF) human can be supplemented with iron or used to bind free iron present in media. It can be used to transport iron into mammalian cells.

Anwendung

apo-Transferrin human has been used as a supplement:

• in Dulbecco′s modified Eagle medium/HamF12 (DMEM/F-12) to culture human adrenocortical carcinoma cells
• in DMEM/F-12 to culture neural precursor cells from mice
• in collecting medium NCSU23 to culture preantral follicles from porcine ovaries

Biochem./physiol. Wirkung

apo-Transferrin (aTF) is an endogenous iron chelator and shows protective effects in the host by scavenging free iron and preventing the formation of toxic reactive oxygen species (ROS). It shows a therapeutic effect against thalassemia. After the release of iron, receptor-bound apo-transferrin reaches the cell surface instead of being transported to lysosomes for degradation. The cell surface has a neutral pH that promotes the release of apo-transferrin from its receptor, where it can again circulate and bind additional iron to undergo further rounds of iron delivery to cells.

Sonstige Hinweise

Nicht-Häm-Eisen-Transportprotein.

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3