SRP3296

WNT-7A human

recombinant, expressed in HEK 293 cells, ≥80% (SDS-PAGE), ≥80% (HPLC)

• Synonym(e): Human WNT-7A, Recombinant WNT-7A, WNT-7A Signaling Molecule
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in HEK 293 cells
• Assay: ≥80% (HPLC); ≥80% (SDS-PAGE)
• Form: lyophilized
• Wirksamkeit: 40-60 ng/mL ED₅₀
• Mol-Gew.: 35.5 kDa
• Verpackung: pkg of 15 μg
• Verunreinigungen: <0.1 EU/μg endotoxin, tested
• Farbe: white
• UniProt-Hinterlegungsnummer: O00755
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... WNT7A(7476)

Beschreibung

Allgemeine Beschreibung

Wnt-7a is expressed in placenta, kidney, testis, uterus, fetal lung and fetal and adult brain. Most Wnt proteins can signal though a mechanism called the canonical Wnt pathway, in which Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of β-Catenein degradation. Intracellular accumulation of β-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors and induces the expression of numerous genes. Increased Wnt/β-Catenein signaling is associated with tumorigenesis in a diverse set of human cancers. However, Wnt-7a/Frizzled-9 signaling has been shown to act as a tumor suppressor in non-small cell lung cancers.

Biochem./physiol. Wirkung

Wnt-7a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. Recombinant human Wnt-7a is a 35.5 kDa glycoprotein containing 318 amino acids. Due to glycosylation, Wnt-7a migrates between 40-55 kDa by SDS-PAGE gel under unreduced conditions

Physikalische Form

Lyophilized with no additives.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of ≤0.2 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable