PP0100

Trypsin-Profil IGD-Kit

• Synonym(e): In gel digestion kit, Proteomics grade Trypsin, TPCK treated
• UNSPSC-Code: 12352200
• NACRES: NA.56

Eigenschaften

• Verwendung: kit sufficient for ≤100 applications
• Qualitätsniveau: 200
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

The Trypsin Profile IGD Kit enables fast, efficient, and complete in-gel tryptic digestion of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS (matrix-assisted laser desorption/ionization-mass spectrometry) and require no additional preparation. Because the Trypsin Profile IGD Kit contains Proteomics Grade Trypsin, a higher sequence coverage and fewer ambiguous autolytic peaks are observed in MALDI spectra.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.

Anwendung

Trypsin Profile IGD Kit has been used for in-gel digestion for LC-MS (liquid chromatography-mass spectrometry)/MS and MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry analysis.

Leistungsmerkmale und Vorteile

• Schnelleres Entfärben als mit alternativen In-Gel Verdau-Kits
• Enthält 100% der Reagenzien für Entfärbung, Verdau und Extraktion der Proteine?/Peptide von Interesse nötig sind
• Kompatibel mit Coomassie, SYPRO^® Orange, SYPRO Ruby, und ProteoSilver^™ gefärbten Gelen
• Die resultierenden Proben sind bereit für die Analyse durch MALDI-MS oder HPLC-MS

Rechtliche Hinweise

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

SYPRO is a registered trademark of Life Technologies

Nur Kit-Komponenten

• Destaining Solution Reconstituted to 75

Sicherheitshinweise

• Piktogramme: GHS02,GHS07,GHS08
• Signalwort: Danger
• H-Sätze: H225 - H302 + H312 + H332 - H315 - H319 - H334 - H335
• P-Sätze: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 3 - Flammable liquids
• WGK: WGK 2
• Flammpunkt (°F): 35.6 °F - closed cup
• Flammpunkt (°C): 2.0 °C - closed cup