P0084

Monoclonal Anti-Pinin antibody produced in mouse

~1.5 mg/mL, clone 5F1, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Anti-DRS, Anti-PNN, Anti-SDK3, Anti-memA
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 5F1, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~140 kDa
• Speziesreaktivität: monkey, bovine, human, canine
• Verpackung: antibody small pack of 25 μL
• Konzentration: ~1.5 mg/mL
• Methode(n): immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 1-2 μg/mL using HeLa nuclear cell extract
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: Q9H307
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PNN(5411)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-Pinin (mouse IgG1 isotype) is derived from the hybridoma 5F1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Pinin (PNN) is a serine/arginine-rich (SR)-related protein, that is found to localize to the cytoplasmic face of the desmosomal plaque in the convergence of intermediate filaments and to pin them to the desmosome. It consists of an Arg-Ser (RS) domain in its carboxyl terminus. This gene is mapped to human chromosome 14q.

Spezifität

Monoclonal Anti-Pinin recognizes human pinin. It crossreacts with monkey, bovine, and dog pinin.

Immunogen

synthetic peptide corresponding to amino acids 547-737 of human pinin, conjugated to KLH.

Anwendung

Monoclonal Anti-Pinin antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

Biochem./physiol. Wirkung

Pinin (PNN) is mainly linked with the mature desmosomes of the epithelia. It plays a role in inducing junction formation and enhance cell aggregation. PNN transcription and protein levels were found to be reduced in renal and transitional cell carcinomas, suggesting its role as a tumor suppressor. PNN protein reduction by RNAi or knockdown resulted in loss of cell-cell adhesion as well as aberrant mouse development. It plays a key role in mRNA export, cell−cell adhesion, alternative splicing and cell migration. PNN also controls gene transcription.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if notused within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.