MAK056

Choline/Acetylcholine Quantification Kit

sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

• Synonym(e): Choline/Acetylcholine Assay Kit
• UNSPSC-Code: 12161503
• NACRES: NA.25

Eigenschaften

• Verwendung: sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)
• Nachweisverfahren: colorimetric; fluorometric
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Choline is an essential nutrient, commonly grouped with the B complex vitamins, that plays key roles in many biological processes. Choline is a precursor for the synthesis of acetylcholine, a critical neurotransmitter in both the peripheral and central nervous systems.

Anwendung

Choline/Acetylcholine Quantification Kit has been used to quantify the amounts of choline and acetylcholine in samples.

Eignung

Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.

Prinzip

In this assay, choline concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λ_ex = 535/λ_em = 587 nm) product, proportional to the choline present. Acetylcholine levels can be determined by adding acetylcholinesterase to the reaction, which hydrolyzes acetylcholine to choline and acetate.

Sicherheitshinweise

• Piktogramme: GHS08,GHS07
• Signalwort: Danger
• H-Sätze: H315,H319,H334,H335
• P-Sätze: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): 188.6 °F - closed cup
• Flammpunkt (°C): 87 °C - closed cup