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Merck

L8402

Sigma-Aldrich

Lysozym aus human neutrophils

≥95% (SDS-PAGE), lyophilized powder, ≥100,000 units/mg protein (E1%/280)

Synonym(e):

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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About This Item

CAS-Nummer:
EC-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54

Biologische Quelle

human neutrophils

Qualitätsniveau

Rekombinant

expressed in E. coli

Assay

≥95% (SDS-PAGE)

Form

lyophilized powder

Spezifische Aktivität

≥100,000 units/mg protein (E1%/280)

Methode(n)

cell based assay: suitable

Eignung

suitable for cell lysis

UniProt-Hinterlegungsnummer

Anwendung(en)

cell analysis

Lagertemp.

−20°C

InChI

1S/C99H159N37O23/c1-11-50(8)77(132-72(139)46-120-81(145)63(32-33-70(101)137)124-88(152)69-31-21-39-136(69)93(157)68(43-73(140)141)131-84(148)62(29-19-37-116-98(109)110)125-90(154)75(48(4)5)135-91(155)76(49(6)7)133-80(144)57(100)24-16-34-113-95(103)104)92(156)126-60(27-17-35-114-96(105)106)82(146)121-51(9)78(142)129-66(41-54-45-119-59-26-15-13-23-56(54)59)87(151)134-74(47(2)3)89(153)122-52(10)79(143)128-65(40-53-44-118-58-25-14-12-22-55(53)58)85(149)123-61(28-18-36-115-97(107)108)83(147)130-67(42-71(102)138)86(150)127-64(94(158)159)30-20-38-117-99(111)112/h12-15,22-23,25-26,44-45,47-52,57,60-69,74-77,118-119H,11,16-21,24,27-43,46,100H2,1-10H3,(H2,101,137)(H2,102,138)(H,120,145)(H,121,146)(H,122,153)(H,123,149)(H,124,152)(H,125,154)(H,126,156)(H,127,150)(H,128,143)(H,129,142)(H,130,147)(H,131,148)(H,132,139)(H,133,144)(H,134,151)(H,135,155)(H,140,141)(H,158,159)(H4,103,104,113)(H4,105,106,114)(H4,107,108,115)(H4,109,110,116)(H4,111,112,117)/t50-,51-,52-,57-,60-,61-,62-,63-,64-,65-,66-,67-,68-,69-,74-,75-,76-,77-/m0/s1

InChIKey

ZJCXKXFAOCLSRV-RYLVIXIQSA-N

Angaben zum Gen

human ... LYZ(4069)

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Allgemeine Beschreibung

Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.

Anwendung

Lysozyme from human neutrophils has been used in the checkerboard method for establishing a synergy between peptidomimetic 1 and immune effector molecules against E. coli.

Biochem./physiol. Wirkung

Lysozymes participates in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.

Einheitendefinition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Physikalische Form

Lyophilized from 50 mM sodium acetate, pH 6.0, with 100 mM NaCl

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

María Victoria Humbert et al.
PLoS pathogens, 13(6), e1006448-e1006448 (2017-07-01)
Pathogenic and commensal Neisseria species produce an Adhesin Complex Protein, which was first characterised in Neisseria meningitidis (Nm) as a novel surface-exposed adhesin with vaccine potential. In the current study, the crystal structure of a recombinant (r)Nm-ACP Type I protein
Maj Hedtjärn et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 24(12), 1333-1351 (2004-12-31)
Brain ischemia triggers an inflammatory reaction that progresses for days to weeks and seems to have a role in secondary progression of injury. Inflammation induces a complex pattern of signaling molecules with partly contradictory actions, and the responses may be
Selectivity in the potentiation of antibacterial activity of alpha-peptide beta-peptoid peptidomimetics and antimicrobial peptides by human blood plasma
Hein-Kristensen L, et al.
Research in Microbiology, 164(9), 933-940 (2013)
Natural Food Antimicrobial Systems (2000)
A A Roger Thompson et al.
Blood, 123(3), 366-376 (2013-11-08)
Neutrophil lifespan and function are regulated by hypoxia via components of the hypoxia inducible factor (HIF)/von Hippel Lindau/hydroxylase pathway, including specific roles for HIF-1α and prolyl hydroxylase-3. HIF-2α has both distinct and overlapping biological roles with HIF-1α and has not

Artikel

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Protokolle

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

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