H9041

Vascular Endothelial Growth Factor 121 human

HumanKine^®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• MDL-Nummer: MFCD00286542
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Produktbezeichnung: Vascular Endothelial Growth Factor 121 human, VEGF121, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: ≤15 ng/mL EC₅₀
• Qualität: endotoxin tested
• Mol-Gew.: dimer 37 kDa (glycosylated); trimer 50 kDa (glycosylated)
• Verpackung: pkg of 10 μg
• Hersteller/Markenname: HumanZyme
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: ≤1 EU/μg
• UniProt-Hinterlegungsnummer: P15692
• Lagertemp.: −20°C
• Angaben zum Gen: human ... VEGFA(7422)

Beschreibung

Allgemeine Beschreibung

HumanPro VEGF-121(vascular endothelial growth factor 121) is an isoform of VEGFA and is expressed in human HEK 293 cells as a disulfide linked, glycosylated cytokine with an apparent molecular mass of 37 kDa as a homodimer and 50 kDa as a homotrimer. VEGF-A exists in three different isoforms VEGF-121, VEGF165 is an angiogenic properties. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
There are five types of VEGF ligands identified in mammals, which exists as different splice variants.VEGFA is a dimeric glycoprotein consisting two monomers antiparallel to each other.

Biochem./physiol. Wirkung

Vascular endothelial growth factor 121 (VEGF121) belongs to the PDGF/VEGF growth factor family. Many cell types secrete VEGF, and it is a potent angiogenic factor and mitogen that stimulates proliferation, migration, and formation of endothelial cells. VEGF stimulates permeabilization of blood vessels and is present in some tumors of the nervous system. VEGF is induced by hypoxia, oncogene mutations, and cytokines such as IL-1, IL-8, and TNF-α.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution of 1X PBS

Hinweis zur Analyse

The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (human umbilical vein endothelial cells).

Rechtliche Hinweise

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable