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GE29-0513-24

HiTrap® SP HP

Cytiva 29-0513-24, pack of 1 mL

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About This Item

UNSPSC-Code:
23151816
NACRES:
NA.56

ligand

sulphopropyl

Beschreibung

Ion Exchanger Type (value)

Verpackung

pack of 1 mL

Hersteller/Markenname

Cytiva 29-0513-24

Verfügbarkeit

not available in North America

Parameter

42 psi

Bettgröße

7 mm × 25 mm

Bettvolumen

1 mL

Säule ID

7 mm

Matrix

6% cross-linked agarose

Partikelgröße

24-44 μm

Durchschnittliche Teilgröße

34 μm

cleaning

3-14

Arbeitsbereich

4-13

Eignung

suitable for bioprocess medium

Allgemeine Beschreibung

HiTrap SP HP are prepacked, ready-to-use SP Sepharose High Performance strong cation exchange columns for high-resolution, small-scale protein purification.

Anwendung

The small particle size (34 μm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose High Performance is a strong cation exchange medium and Q Sepharose High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.

Leistungsmerkmale und Vorteile

  • 34 μm bead size for high-performance, high-resolution purifications.
  • Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
  • Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
  • Strong sulfopropyl (SP) cation exchanger.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Sonstige Hinweise

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Rechtliche Hinweise

HiTrap is a registered trademark of Cytiva

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Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

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Artikel

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Alle anzeigen

Protokolle

Refolding using ion exchange chromatography and analysis of refolding

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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