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GE17-1408-01

HiTrap® Desalting Columns

Cytiva 17-1408-01, pack of 5 × 5 mL

Synonym(e):

Purification Columns

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About This Item

UNSPSC-Code:
23151816
NACRES:
NA.56

Verpackung

pack of 5 × 5 mL

Hersteller/Markenname

Cytiva 17-1408-01

Lagerbedingungen

dry media

Parameter

42 psi

Bettgröße

16 mm × 25 mm

Bettvolumen

5 mL

Säule ID

16 mm

Matrix

cross-linked dextran

Partikelgröße

>20 μm (dry)

cleaning

2-13

working range

2-13

Eignung

suitable for bioprocess medium

Verwandte Kategorien

Allgemeine Beschreibung

HiTrap® Desalting is a ready-to-use 5 mL column, prepacked with Sephadex® G-25 Superfine, for fast and convenient desalting and buffer exchange.

HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.

Anwendung

Desalting and buffer exchange with Sephadex® G-25.

Leistungsmerkmale und Vorteile

  • Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
  • Replaces dialysis as the method of choice for desalting.
  • Desalting requires less than one minute due to the high rigidity of Sephadex® G-25 and the optimized packing technology.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sonstige Hinweise

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Rechtliche Hinweise

HiTrap is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

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Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

General Considerations for Purification of GST-tagged Protein

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

Performing a purification and on-column refolding of an insoluble histidine-tagged protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

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Protokolle

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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