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GE17-5087-02

Sigma-Aldrich

HiPrep 26/10 Desalting

Cytiva 17-5087-02, column L × I.D. 10 cm × 26 mm, 17-132 μm particle size (dry)

Synonym(e):

Desalting Column, HiPrep Desalting Column

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About This Item

UNSPSC-Code:
41115700
NACRES:
SB.52

Materialien

polypropylene (PP)

Mol-Gew.

1000-5000 Da (globular proteins)

Verpackung

pack of 4

Hersteller/Markenname

Cytiva 17-5087-02

Lagerbedingungen

dry media

Parameter

<15 mL sample volume
<31 mL/min flow rate
0.15 Mpa (22 psi)

Bettvolumen

53 mL

Säule L × ID

10 cm × 26 mm

Matrix

cross-linked dextran
polymeric

Aktive Matrixgruppe

dextran-epichlorohydrin phase

Partikelgröße

17-132 μm (dry)

cleaning

2-13

working range

2-13

Trenntechnik

size exclusion (SEC)

Allgemeine Beschreibung

HiPrep 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.

Leistungsmerkmale und Vorteile

  • Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
  • Replaces dialysis for desalting and therefore saves time and reduces labor.
  • Compatible with a laboratory pump or chromatography system such as AKTA design.
  • Two or four columns can be used in series for sample sizes of 30 to 60 mL.

Eignung

All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Rechtliche Hinweise

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Lagerklassenschlüssel

3 - Flammable liquids


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

This page describes automated purification of antibodies using ÄKTA chromatography systems.

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

Alle anzeigen

Protokolle

AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

This page how to remove histidine tags by enzymatic cleavage using GE Healthcare products.

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

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