GE17-0300-50
Ficoll® PM 400
Cytiva 17-0300-50, pack of 500 g
Synonym(e):
Ficoll Density Gradient
About This Item
Empfohlene Produkte
Form
solid
spray-dried
Verpackung
pack of 500 g
Hersteller/Markenname
Cytiva 17-0300-50
Farbe
White
Löslichkeit
soluble
Allgemeine Beschreibung
Ficoll® PM400 is a synthetic neutral, highly-branched hydrophilic polymer of sucrose with an average molecular weight of 400 000. It has long been used to form density gradients for separating and isolating eukaryotic cells, organelles and bacterial cells, as a stabilizing agent, and as a preparation medium for isolating mononuclear cells. Applications can also be found in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies.
Anwendung
It also has applications in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies.
Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity.
Leistungsmerkmale und Vorteile
- For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
- Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
- Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
- Better osmotic properties than sucrose.
- Preserves functional and morphological integrity.
- Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
- Does not penetrate biological membranes.
- Serves as the raw material for preparation of Ficoll-Paque gradients.
- Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Lagerklassenschlüssel
13 - Non Combustible Solids
Analysenzertifikate (COA)
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Artikel
Removal of Percoll after centrifugation
The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.
This page shows How to fractionate and analyze gradients of Percoll
The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.
Protokolle
The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.
How to use percoll to prepare a gradient
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