E6406

EZview^™ Red Glutathione Affinity Gel

• MDL-Nummer: MFCD05665380
• UNSPSC-Code: 41106500
• NACRES: NA.56

Eigenschaften

• chemische Klasse(n) des Analyten: proteins (GST)
• Qualitätsniveau: 200
• Methode(n): immunoprecipitation (IP): suitable; protein purification: suitable
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

EZviewä Red Glutathione Affinity Gel is a highly visible, red colored glutathione agarose affinity gel. The affinity resin is composed of glutathione covalently attached through the sulfur to epoxy-activated 4% cross-linked agarose beads, resulting in a 12-atom spacer.

Anwendung

The product is used for affinity capture, molecular pull-down, or IP of proteins containing glutathione binding sequences, such as glutathione S-transferase, glutathione peroxidase and glyoxalase I.

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview^™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview^™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Leistungsmerkmale und Vorteile

• Increased visibility - Red color reduces risk of incidental aspiration
• Improved recovery of target protein by reduced accidental loss
• Higher reproducibility - More consistent yields

Physikalische Form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Rechtliche Hinweise

EZview is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3