RCE

95081046, rabbit eye, Epithelial-like

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: RCE, 95081046
• Biologische Quelle: rabbit eye
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rabbit corneal epithelial cells, SV40 transformed

Beschreibung der Zelllinie

The RCE cell line, also known as K′RCE, was established after infection of primary cultured rabbit (New Zealand albino) corneal epithelial cells with a recombinant SV40 vector. The cells exhibit a cobblestone-like appearance and stratification, including desmosome formation and microvilli development. Expression of keratin AE1 was always detectable whereas AE5 was expressed only at confluency.

Anwendung

Corneal epithelial research

Nährmedium

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5μg/ml Insulin + 10ng/ml EGF + 0.1μg/ml Cholera toxin + 0.5% Dimethyl Sulphoxide (DMSO) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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