C2-Rev 7

94101907, rat liver, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: C2-Rev 7, 94101907
• Biologische Quelle: rat liver
• Wachstumsmodus: Adherent
• Karyotyp: Hyperdiploid, 51 chromosomes
• Morphologie: Epithelial
• Produkte: Albumin and hepatocyte markers
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat hepatoma [HGPRT-]

Beschreibung der Zelllinie

C2-Rev 7 is a revertant of the C2 variants, selected by virtue of its capacity to grow in glucose free medium (containing dialysed serum) where survival requires activity of the liver-specific gluconeogenic pathway. Expression of the entire set of liver specific functions is restored. Albumin and hepatocyte markers present.

Anwendung

Gene expression studies.

Nährmedium

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split semi-confluent cultures (70%) 1:10 i.e. seeding at 9x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1,000,000 cells/10cm² dish.

Sonstige Hinweise

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